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The dual action of probiotic lactobacilli in suppressing virulence and survival of Arcobacter butzleri

2025-04-14Artigo científico Acesso restrito
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dc.contributor.authorVieira, Alexandre
dc.contributor.authorMateus, Cristiana
dc.contributor.authorFonseca, Inês M.
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorOleastro, Mónica
dc.contributor.authorFerreira, Susana
dc.date.accessioned2026-01-22T09:54:59Z
dc.date.available2026-01-22T09:54:59Z
dc.date.issued2025-04-14
dc.description.abstractArcobacter butzleri is a widely distributed foodborne and waterborne pathogen, increasingly recognized as an emerging enteropathogen. Understanding its survival mechanisms and interactions with probiotics is crucial for developing targeted intervention strategies. A. butzleri must withstand various hostile conditions to successfully colonize the gastrointestinal tract, including inhibition by probiotics, such as Limosilactobacillus reuteri, Lactobacillus acidophilus and Lactiplantibacillus plantarum. Thus, this study aimed to assess the survival of A. butzleri under acidic conditions and determine its minimum inhibitory concentration (MIC) for bile salts. Additionally, the antimicrobial potential of the lactobacilli strains was evaluated by analysing the effects of their culture-free supernatant (CFS) on A. butzleri growth, coculture interactions, and biofilm formation. The influence of lactobacilli on A. butzleri was further investigated through competition, displacement and exclusion assays using Caco-2 cell models. The results indicate that lactobacilli strains exhibit tolerance to acidic environments and physiological bile salt concentrations, whereas A. butzleri was more susceptible to acidic stress. The antagonistic effect of lactobacilli was evidenced by growth inhibition of A. butzleri in the presence of CFS or during coculture. However, CFS from certain lactobacilli strains was found to enhance biofilm formation, highlighting potential consequences. Furthermore, while lactobacilli did not demonstrate significant ntagonistic effects in competition assays, they effectively displaced and excluded A. butzleri in the Caco-2 infection model. Overall, these findings suggest that probiotic lactobacilli can inhibit A. butzleri growth, yet their impact on its virulence remains uncertain. This underscores the need for strain-specific probiotic selection to effectively target this pathogen and emphasizes that not every probiotic contribute to the prevention of A. butzleri infections.eng
dc.description.abstractHighlights: - Lactobacilli inhibit A. butzleri growth via CFS or during coculture interactions. - L. plantarum CFS promoted A. butzleri biofilm formation. - None of the probiotic strains influenced the adhesion of A. butzleri to Caco-2 cells. - Lactobacilli displaced and excluded A. butzleri from Caco-2 cells.eng
dc.description.sponsorship((This work was developed within the scope of the CICS-UBI projects UIDB/00709/2020 and UIDP/00709/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES. Cristiana Mateus is recipient of a doctoral fellowship (UI/BD/151023/2021) under the scope of the CICS-UBI Programmatic Funding (UIDP/00709/2020).))
dc.identifier.citationMicrob Pathog. 2025 Jul:204:107589. doi: 10.1016/j.micpath.2025.107589. Epub 2025 Apr 14
dc.identifier.doi10.1016/j.micpath.2025.107589
dc.identifier.eissn1096-1208
dc.identifier.issn0882-4010
dc.identifier.pmid40239725
dc.identifier.urihttp://hdl.handle.net/10400.18/10735
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationUnravelling the virulence potential of Aliarcobacter butzleri
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0882401025003146?via%3Dihub#sec7
dc.relation.ispartofMicrobial Pathogenesis
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntagonistic Effect
dc.subjectAntimicrobial Activity
dc.subjectArcobacter Butzleri
dc.subjectBiofilm Formation
dc.subjectCell Infection
dc.subjectLactobacilli
dc.subjectInfecções Gastrointestinais
dc.titleThe dual action of probiotic lactobacilli in suppressing virulence and survival of Arcobacter butzlerieng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleUnravelling the virulence potential of Aliarcobacter butzleri
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F151023%2F2021/PT
oaire.citation.startPage107589
oaire.citation.titleMicrobial Pathogenesis
oaire.citation.volume204
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
relation.isProjectOfPublicatione0fbaa7c-391a-479d-9bcf-efb7997d4004
relation.isProjectOfPublication60f6a70b-110d-4c15-a3fd-d43d078078bc
relation.isProjectOfPublication.latestForDiscoveryced21566-cfde-4640-afff-0536a07c1f8f

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