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Maize IgE binding proteins: each plant a different profile?
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Orientador(es)
Resumo(s)
Background: Allergies are nearly always triggered by protein molecules and the majority of individuals with documented
immunologic reactions to foods exhibit IgE hypersensitivity reactions. In this study we aimed to understand if natural
differences, at proteomic level, between maize populations, may induce different IgE binding proteins profiles among
maize-allergic individuals. We also intended to deepen our knowledge on maize IgE binding proteins.
Results: In order to accomplish this goal we have used proteomic tools (SDS-PAGE and 2-D gel electrophoresis followed
by western blot) and tested plasma IgE reactivity from four maize-allergic individuals against four different protein fractions
(albumins, globulins, glutelins and prolamins) of three different maize cultivars. We have observed that maize cultivars have
different proteomes that result in different IgE binding proteins profiles when tested against plasma from maize-allergic
individuals. We could identify 19 different maize IgE binding proteins, 11 of which were unknown to date. Moreover, we
found that most (89.5%) of the 19 identified potential maize allergens could be related to plant stress.
Conclusions: These results lead us to conclude that, within each species, plant allergenic potential varies with genotype.
Moreover, considering the stress-related IgE binding proteins identified, we hypothesise that the environment, particularly
stress conditions, may alter IgE binding protein profiles of plant components.
Descrição
Palavras-chave
Segurança Alimentar Maize Allergens Western-blot 2D-gel Electrophoresis Maize Proteins
Contexto Educativo
Citação
Proteome Sci. 2014 Mar 20;12(1):17. doi: 10.1186/1477-5956-12-17
Editora
BioMed Central