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Ingested nanomaterials: impact of digestion process in the physicochemical characteristics and biological consequences in intestinal cells

2021-11-11Conference object Restricted access
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dc.contributor.authorVieira, Adriana
dc.contributor.authorVital, Nádia
dc.contributor.authorRoque, Rossana
dc.contributor.authorGramacho, Ana Catarina
dc.contributor.authorRolo, Dora
dc.contributor.authorGonçalves, Lídia D.
dc.contributor.authorBettencourt, Ana
dc.contributor.authorMartins, Carla
dc.contributor.authorAssunção, Assunção
dc.contributor.authorAlvito, Paula
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2022-01-10T16:34:28Z
dc.date.available2022-01-10T16:34:28Z
dc.date.issued2021-11-11
dc.description.abstractNanomaterials(NMs) provide a basis for key enabling technologies, in view of their potential to improve many products and processes, namely in food and feed industry. That is the case of titanium dioxide NMs(TiO2 NMs), presenting beneficial properties for a broad range of innovative applications such as food additives, toothpaste, pharmaceuticals, food products, etc., that may drive ingestion. The oral exposure can occur directly, by consumption of products/pharmaceuticals or foods containing NMs, or indirectly, through the ingestion of foods contaminated with NMs released from food-contact materials or environmental sources. As such, the gastrointestinal tract is the first site of contact of the ingested NMs, allowing a systemic exposure if the intestinal barriers is surpassed. This work aimed to investigate how the digestion process affects the physicochemical properties of three different TiO2 NMs(NM-102, NM-103 and NM-105) and their toxic effects on intestinal cells. After undergoing digestion through the standardized static INFOGEST 2.0 in vitro digestion method, the cytotoxicity of the TiO2 NMs was determined in Caco-2 and HT29-MTX-E12 intestinal cells, using the MTT assay. Furthermore, the cytokinesis-blocked micronucleus assay was used to investigate their genotoxicity in both cell lines in order to predict their carcinogenic potential. The results showed that, for one TiO2 NM(NM-105), the digestion caused changes in the hydrodynamic size of the NM and a more pronounced toxicity in HT29-MTX-E12 intestinal cells, as compared to the undigested one. The micronucleus assay suggests effects on the chromosomal integrity in the HT29-MTXE12 cells, for all the tested TiO2 NM especially after the in vitro digestion. Overall, we conclude that including the digestion prior to the in vitro bioassays for the safety evaluation of ingested NMs, allows integrating the physiological modifications that the NMs suffer in the organism, contributing to an improved hazard assessment of ingested NMs.pt_PT
dc.description.sponsorshipThe authors acknowledge Joint Research Centre(JRC, Ispra, Italy) for providing the NMs and funding by the Portuguese Foundation for Science and Technology FCT/MCTES through national funds(PIDDAC), PTDC/SAU-PUB/29481/2017, Toxicogenomics(UIDB/00009/2020; UIDP/00009/2020), iMed.ULisboa(UIDB/04138/2020 and UIDP/04138/2020) and CESAM(UIDP/50017/2020+UIDB/50017/2020. L. Gonçalves Principal Researcher grant (CEECIND/03143/2017). FCT/MCTES PhD studentship 2020.07168.BD was attributed to NV.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7844
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCEECIND/03143/2017pt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectIngested Nanomaterialspt_PT
dc.subjectNanotoxicologypt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectNanomaterialspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectSegurança Alimentar
dc.subjectComposição dos Alimentos
dc.subjectAvaliação do Risco
dc.titleIngested nanomaterials: impact of digestion process in the physicochemical characteristics and biological consequences in intestinal cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.citation.conferencePlace(online)/ Braga, Portugalpt_PT
oaire.citation.titleTOTAL NANOSAFE: from molecules to public health,11-12 november 2021pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
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oaire.fundingStream6817 - DCRRNI ID
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oaire.fundingStream6817 - DCRRNI ID
person.familyNameAlvito
person.familyNameSilva
person.familyNameLouro
person.givenNamePaula
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier125777
person.identifier157627
person.identifier.ciencia-id3B14-020B-5FB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0001-8396-1357
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.ridB-6977-2014
person.identifier.scopus-author-id8590147800
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
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