Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications

Subtil, Joao; Bajanca-Lavado, Maria Paula; Rodrigues, Joao; Duarte, Aida; Reis, Lucia; Nogueira, Isabel; Jordão, Luisa

Publication

Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications

2018-10-01Journal article

dc.contributor.author	Subtil, Joao
dc.contributor.author	Bajanca-Lavado, Maria Paula
dc.contributor.author	Rodrigues, Joao
dc.contributor.author	Duarte, Aida
dc.contributor.author	Reis, Lucia
dc.contributor.author	Nogueira, Isabel
dc.contributor.author	Jordão, Luisa
dc.date.accessioned	2019-02-15T14:05:59Z
dc.date.available	2019-02-15T14:05:59Z
dc.date.issued	2018-10-01
dc.description.abstract	INTRODUCTION: Adenoids are nasopharyngeal lymphoid tissue with a relevant role in host defence against infection of upper respiratory tract. Nevertheless, adenoids are also a reservoir of microorganisms that can cause infections of upper respiratory tract and otitis particularly in children. OBJECTIVE: Evaluate and compare the association between biofilm assembly on adenoids and the incidence of recurrent infections in a paediatric population submitted to adenoidectomy by either infectious or non-infectious indication. METHODS: Scanning electron microscopy was used to assess biofilms on adenoid surface; biofilm assembly in vitro was monitored by crystal violet assay; antibiotic susceptibility was assessed following EUCAST guidelines; Hinfluenzae capsular typing was performed by PCR. RESULTS: Biofilms were present in 27.4% of adenoid samples and no statistical difference was found between infectious and non-infectious groups. In vitro, the most clinically relevant bacteria, H.influenzae, S.aureus, S.pyogenes, S.pneumoniae and M.catarrhalis, were mostly moderate biofilm assemblers (71.7%). 55.3% of these bacteria were intermediate/resistant to at least one of the tested antibiotics. No association was found between the ability to assemble biofilms in vitro and the presence of biofilms on adenoids nor antibiotic resistance. All H.influenzae were characterized as non-typeable. CONCLUSION: The presence of biofilms on adenoid surface was independent from clinical sample background. Bacterial ability to assemble biofilms in vitro cannot be used to predict biofilm assembly in vivo. The lack of correlation between biofilm formation and infectious respiratory diseases found contributes to question the relevance of biofilms on the pathogenesis of infectious diseases.	pt_PT
dc.description.sponsorship	National Institutes of Health [grant numbers 2015DDI1143, 2015	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Otolaryngol Pol. 2019; 73(1):22-28. Epub 2018 Oct 01. doi:10.5604/01.3001.0012.5278	pt_PT
dc.identifier.doi	10.5604/01.3001.0012.5278	pt_PT
dc.identifier.issn	0030-6657
dc.identifier.uri	http://hdl.handle.net/10400.18/5827
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Elsevier/ Polish Otorhinlaryngology - Head and Neck Surgery Society	pt_PT
dc.relation.publisherversion	https://otolaryngologypl.com/resources/html/article/details?id=179800&language=en	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	pt_PT
dc.subject	Adenoid	pt_PT
dc.subject	Biofilms	pt_PT
dc.subject	Scanning Electron Microscopy	pt_PT
dc.subject	Haemophilus	pt_PT
dc.subject	Children	pt_PT
dc.subject	Infecções Respiratórias
dc.subject	Ar e Saúde Ocupacional
dc.title	Prospective observational study of adenoidal biofilms in a paediatric population and their clinical implications	pt_PT
dc.title.alternative	Cross-sectional study of adenoidal biofilms in a paediatric population and its clinical implications
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	28	pt_PT
oaire.citation.issue	5	pt_PT
oaire.citation.startPage	22	pt_PT
oaire.citation.title	Otolaryngologia Polska	pt_PT
oaire.citation.volume	72	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT