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Simultaneous quantitation of sphingoid bases by UPLC-ESI-MS/MS with identical13C-encoded internal standards

2017-03Journal article Restricted access
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dc.contributor.authorMirzaian, M.
dc.contributor.authorWisse, P.
dc.contributor.authorFerraz, M.J.
dc.contributor.authorMarques, A.R.A.
dc.contributor.authorGaspar, P.
dc.contributor.authorOussoren, S.V.
dc.contributor.authorKytidou, K.
dc.contributor.authorCodée, J.D.C.
dc.contributor.authorvan der Marel, G.
dc.contributor.authorOverkleeft, H.S.
dc.contributor.authorAerts, J.M.
dc.date.accessioned2018-03-22T19:28:45Z
dc.date.available2018-03-22T19:28:45Z
dc.date.issued2017-03
dc.description.abstractFree sphingoid bases (lysosphingolipids) of primary storage sphingolipids are increased in tissues and plasma of several sphingolipidoses. As shown earlier by us, sphingoid bases can be accurately quantified using UPLC-ESI-MS/MS, particularly in combination with identical13C-encoded internal standards. The feasibility of simultaneous quantitation of sphingoid bases in plasma specimens spiked with a mixture of such standards is here described. The sensitivity and linearity of detection is excellent for all examined sphingoid bases (sphingosine, sphinganine, hexosyl-sphingosine (glucosylsphingosine), hexosyl2-sphingosine (lactosylsphingosine), hexosyl3-sphingosine (globotriaosylsphingosine), phosphorylcholine-sphingosine) in the relevant concentration range and the measurements show very acceptable intra- and inter-assay variation (<10% average). Plasma samples of a series of male and female Gaucher Disease and Fabry Disease patients were analyzed with the multiplex assay. The obtained data compare well to those earlier determined for plasma globotriaosylsphingosine and glucosylsphingosine in GD and FD patients. The same approach can be also applied to measure sphingolipids in the same sample. Following extraction of sphingolipids from the same sample these can be converted to sphingoid bases by microwave exposure and subsequently quantified using13C-encoded internal standards.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationClin Chim Acta. 2017 Mar;466:178-184. doi: 10.1016/j.cca.2017.01.014. Epub 2017 Jan 13.pt_PT
dc.identifier.doi10.1016/j.cca.2017.01.014pt_PT
dc.identifier.issn0009-8981
dc.identifier.urihttp://hdl.handle.net/10400.18/5462
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0009898117300256?via%3Dihubpt_PT
dc.subjectCarbon Isotopespt_PT
dc.subjectChromatography, High Pressure Liquidpt_PT
dc.subjectFabry Diseasept_PT
dc.subjectFemalept_PT
dc.subjectGaucher Diseasept_PT
dc.subjectHumanspt_PT
dc.subjectMalept_PT
dc.subjectReference Standardspt_PT
dc.subjectSphingolipidosespt_PT
dc.subjectSphingolipidspt_PT
dc.subjectTandem Mass Spectrometrypt_PT
dc.subjectDoenças Genéticaspt_PT
dc.titleSimultaneous quantitation of sphingoid bases by UPLC-ESI-MS/MS with identical13C-encoded internal standardspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage184pt_PT
oaire.citation.startPage178-184pt_PT
oaire.citation.titleClinica Chimica Actapt_PT
oaire.citation.volume466pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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