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Insights into the population structure and pan-genome of Haemophilus influenzae

2018-10-31Journal article Restricted access
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dc.contributor.authorPinto, M.
dc.contributor.authorGonzález-Díaz, A.
dc.contributor.authorMachado, M.P.
dc.contributor.authorDuarte, S.
dc.contributor.authorVieira, L.
dc.contributor.authorCarriço, J.A.
dc.contributor.authorMarti, S.
dc.contributor.authorBajanca-Lavado, M.P.
dc.contributor.authorGomes, J.P.
dc.date.accessioned2019-03-21T14:48:37Z
dc.date.available2019-03-21T14:48:37Z
dc.date.issued2018-10-31
dc.descriptionSupplementary data to this article can be found online at https:// doi.org/10.1016/j.meegid.2018.10.025.pt_PT
dc.description.abstractThe human-restricted bacterium Haemophilus influenzae is responsible for respiratory infections in both children and adults. While colonization begins in the upper airways, it can spread throughout the respiratory tract potentially leading to invasive infections. Although the spread of H. influenzae serotype b (Hib) has been prevented by vaccination, the emergence of infections by other serotypes as well as by non-typeable isolates (NTHi) have been observed, prompting the need for novel prevention strategies. Here, we aimed to study the population structure of H. influenzae and to get some insights into its pan-genome. We studied 305H. influenzae strains, enrolling 217 publicly available genomes, as well as 88 newly sequenced H. influenzae invasive strains isolated in Portugal, spanning a 24-year period. NTHi isolates presented a core-SNP-based genetic diversity about 10-fold higher than the one observed for Hib. The analysis of key factors involved in pathogenesis, such as lipooligosaccharides, hemagglutinating pili and High Molecular Weight-adhesins, suggests that NTHi shape its virulence repertoire, either by acquisition and loss of genes or by SNP-based diversification, likely towards host immune evasion and persistence. Discreet NTHi subpopulations structures are proposed based on core-genome supported with 17 candidate genetic markers identified in the accessory genome. Additionally, this study provides two bioinformatics tools for in silico rapid identification of H. influenzae serotypes and NTHi clades previously proposed, obviating laboratory-based demanding procedures. The present study constitutes an important genomic framework that could lay way for future studies on the genetic determinants underlying invasiveness and disease and population structure of H. influenzae.pt_PT
dc.description.sponsorshipThis work was supported by GenomePT (ref. POCI-01-0145-FEDER022184) from Fundação para a Ciência e Tecnologia, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInfect Genet Evol. 2019 Jan;67:126-135. doi: 10.1016/j.meegid.2018.10.025. Epub 2018 Oct 31.pt_PT
dc.identifier.doi10.1016/j.meegid.2018.10.025pt_PT
dc.identifier.issn1567-1348
dc.identifier.urihttp://hdl.handle.net/10400.18/6267
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1111/bjh.15600pt_PT
dc.subjectHaemophilus Influenzaept_PT
dc.subjectNon-typeablept_PT
dc.subjectPan-genomept_PT
dc.subjectPathogenesispt_PT
dc.subjectWhole-genome Sequencingpt_PT
dc.subjectGenomicspt_PT
dc.subjectComputational Biology
dc.subjectGenetic Variation
dc.subjectHaemophilus Infections
dc.subjectHumans
dc.subjectPhylogeny
dc.subjectPolymorphism, Single Nucleotide
dc.subjectVirulence
dc.subjectGenome, Bacterial
dc.subjectInfecções Respiratórias
dc.titleInsights into the population structure and pan-genome of Haemophilus influenzaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage135pt_PT
oaire.citation.startPage126pt_PT
oaire.citation.titleInfection, Genetics and Evolutionpt_PT
oaire.citation.volume67pt_PT
rcaap.embargofctPolítica editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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