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Screening of Bacteriocinogenic Lactic Acid Bacteria and Their Characterization as Potential Probiotics

2020-03-11Journal article Open access
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dc.contributor.authorPinto, Ana
dc.contributor.authorBarbosa, Joana
dc.contributor.authorAlbano, Helena
dc.contributor.authorIsidro, Joana
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2021-04-01T14:21:22Z
dc.date.available2021-04-01T14:21:22Z
dc.date.issued2020-03-11
dc.description.abstractProbiotics are living microorganisms used as nutritional additives that confer health benefits on the host. Their use in food products is very attractive, especially if they could also inhibit important foodborne pathogens. In this study, antimicrobial activity against several foodborne pathogens was screened for 280 lactic acid bacteria (LAB) isolated from different food products and the probiotic characteristics of bacteriocinogenic isolates were evaluated. Seven out of 280 LAB isolates were selected due to their bacteriocinogenic properties and identified by 16S rRNA gene sequence analysis as Pediococcuspentosaceus (n = 6) and Lactobacillus plantarum (n = 1). Virulence factors and antibiotic resistances were not detected for any of the isolates. Except for L. plantarum R23, all the isolates were able to survive through the simulated gastrointestinal tract conditions. Only P.pentosaceus CFF4 was able to adhere to Caco-2 cells after the simulated gastrointestinal tract passage. In conclusion, even though in vivo studies should be performed, P.pentosaceus CFF4, which was also able to inhibit the growth of foodborne pathogens in vitro, seems to be a potential probiotic to be used in the food industry.pt_PT
dc.description.sponsorshipThis research was funded by NEWFOOD—Food Technologies Valorization (NORTE-01-0246-FEDER-000043) that is co-funded by the Regional Operational Program of the North (Norte2020), under the PORTUGAL 2020 Partnership Agreement, through the implementation of the European Regional Development Fund (ERFD). The authors are grateful to Paul Gibbs for the English edition. We would also like to thank the scientific collaboration under the Fundação para a Ciência e Tecnologia (FCT) project UID/Multi/50016/2019. Financial support for author Joana Barbosa was also provided by FCT through post-doctoral grant SFRH/BPD/113303/2015.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2020 Mar 11;8(3):393. doi: 10.3390/microorganisms8030393pt_PT
dc.identifier.doi10.3390/microorganisms8030393pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/7622
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.relationClostridium difficile: prevalence in foods and study of alternative treatments to conventional therapeutics applied on C. difficile infection
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/8/3/393pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial Activitypt_PT
dc.subjectBacteriocinpt_PT
dc.subjectLactic Acid Bacteriapt_PT
dc.subjectProbioticpt_PT
dc.titleScreening of Bacteriocinogenic Lactic Acid Bacteria and Their Characterization as Potential Probioticspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardTitleClostridium difficile: prevalence in foods and study of alternative treatments to conventional therapeutics applied on C. difficile infection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F113303%2F2015/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage393pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication62ad761e-6507-4e6d-9064-a3d3fb9c57ad
relation.isProjectOfPublication2fc319ad-c2cd-48ff-afc1-a6228fa05c57
relation.isProjectOfPublication.latestForDiscovery62ad761e-6507-4e6d-9064-a3d3fb9c57ad

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