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Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression

2022-02-26Journal article Open access
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dc.contributor.authorTanoeiro, Luís
dc.contributor.authorOleastro, Mónica
dc.contributor.authorNunes, Alexandra
dc.contributor.authorMarques, Andreia T.
dc.contributor.authorDuarte, Sílvia Vaz
dc.contributor.authorGomes, João Paulo
dc.contributor.authorMatos, António Pedro Alves
dc.contributor.authorVítor, Jorge M.B.
dc.contributor.authorVale, Filipa F.
dc.date.accessioned2023-01-31T14:27:40Z
dc.date.available2023-01-31T14:27:40Z
dc.date.issued2022-02-26
dc.descriptionThis article belongs to the Special Issue Bacteriophage Genomics.pt_PT
dc.description.abstractCampylobacter coli and C. jejuni, the causing agents of campylobacteriosis, are described to be undergoing introgression events, i.e., the transference of genetic material between different species, with some isolates sharing almost a quarter of its genome. The participation of phages in introgression events and consequent impact on host ecology and evolution remain elusive. Three distinct prophages, named C. jejuni integrated elements 1, 2, and 4 (CJIE1, CJIE2, and CJIE4), are described in C. jejuni. Here, we identified two unreported prophages, Campylobacter coli integrated elements 1 and 2 (CCIE1 and CCIE2 prophages), which are C. coli homologues of CJIE1 and CJIE2, respectively. No induction was achieved for both prophages. Conversely, induction assays on CJIE1 and CJIE2 point towards the inducibility of these prophages. CCIE2-, CJIE1-, and CJIE4-like prophages were identified in a Campylobacter spp. population of 840 genomes, and phylogenetic analysis revealed clustering in three major groups: CJIE1-CCIE1, CJIE2-CCIE2, and CJIE4, clearly segregating prophages from C. jejuni and C. coli, but not from human- and nonhuman-derived isolates, corroborating the flowing between animals and humans in the agricultural context. Punctual bacteriophage host-jumps were observed in the context of C. jejuni and C. coli, and although random chance cannot be fully discarded, these observations seem to implicate prophages in evolutionary introgression events that are modulating the hybridization of C. jejuni and C. coli species.pt_PT
dc.description.sponsorshipF.F.V. is funded by Fundação para a Ciência e a Tecnologia (FCT) through an Assis tant Researcher grant CEECIND/03023/2017, and a project grant (PTDC/BTM-SAL/28978/2017) that supported this work. The work is partially supported by Na-tional funds from FCT, projects UIDB/04138/2020 and UIDP/04138/2020. Campylobacter strains were sequenced under the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalization (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Oper-ational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by FCT. This work was also supported by Fundos FEDER through the Programa Op eracional Factores de Competitivida-de—COMPETE and by Fundos Nacionais through the FCT within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health-ToxOmics).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2022 Feb 26;10(3):516. doi: 10.3390/microorganisms10030516.pt_PT
dc.identifier.doi10.3390/microorganisms10030516pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/8484
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationNot Available
dc.relationPhage-Enzybiotic: dealing with critical pathogenic antibiotic-resistant bacteria
dc.relationResearch Institute for Medicines
dc.relationCentre for Toxicogenomics and Human Health
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/10/3/516pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCampylobacterpt_PT
dc.subjectBacteriophagept_PT
dc.subjectHost Rangept_PT
dc.subjectIntrogressionpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleCryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgressionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardTitlePhage-Enzybiotic: dealing with critical pathogenic antibiotic-resistant bacteria
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03023%2F2017%2FCP1476%2FCT0004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F28978%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F00009%2F2019/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage516pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamCEEC IND 2017
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationbe784e75-da77-4fcf-8af2-53b342fd4db1
relation.isProjectOfPublicatione76f78dc-058d-47bd-a8e4-b555d358bb14
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