Repository logo
 
Publication

Validation of an HPLC-DAD/UV method for the quantification of cyclamate in tabletop sweeteners: risk of exceeding the acceptable daily intake

2017-06Journal article Restricted access
Simple item page
dc.contributor.authorVasco, Elsa
dc.contributor.authorSargaço, B.
dc.contributor.authorSerra, C.
dc.date.accessioned2018-03-06T19:32:29Z
dc.date.available2018-03-06T19:32:29Z
dc.date.issued2017-06
dc.description.abstractCyclamate is widely used as intense sweetener in the European Union. The absence of a maximum limit for the use of cyclamate in tabletop sweeteners and the growing demand for this type of product highlights the importance of developing robust analytical methods for the determination of its content to understand if the consumption of tabletop sweeteners can have a negative impact on human health. The present work aimed at the optimisation and validation of an high-performance liquid chromatography (HPLC) analytical method for cyclamate determination in tabletop sweeteners based on the procedure of European Standard EN 12857. The validated method was then applied to the determination of this sweetener in different types of tabletop sweeteners (liquid, powder and tablets). Both standards and samples solutions were submitted to a derivatisation procedure which converted cyclamate to N,N-dichlorocyclohexylamine. The derivatised product was separated and quantified using a reversed-phase column, a mobile phase composed of water (20%) and methanol (80%), isocratic flow of 1 ml min-1, and detection by ultraviolet spectrophotometry at a wavelength of 314 nm. The analytical method was internally validated according to the following validation parameters: working range, linearity, limits of detection and quantification, sensitivity, precision (repeatability and intermediate precision), and uncertainty. This method proved to be specific and selective for the determination of this sweetener, showing repeatability, RSDr ≤ 3%, intermediate precision, RSDR ≤ 3.3%, and recovery rates from 92% to 108% for the different tabletop sweeteners. The method uncertainty was 9.4%. The concentration of cyclamate in the samples varied significantly, from 2.9% to 73.9%, which demonstrated that a possible excessive consumption of one of the analysed sweeteners can lead to exceeding the acceptable daily intake for cyclamate.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Addit Contam Part A: Chem Anal Control Expo Risk Assess. 2017 Jun;34(6):883-890. doi: 10.1080/19440049.2017.1306756. Epub 2017 Apr 11.pt_PT
dc.identifier.doi10.1080/19440049.2017.1306756pt_PT
dc.identifier.issn1944-0049 (print)
dc.identifier.issn1944-0057 (online)
dc.identifier.urihttp://hdl.handle.net/10400.18/5269
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/19440049.2017.1306756pt_PT
dc.subjectCyclamatept_PT
dc.subjectADIpt_PT
dc.subjectHPLCpt_PT
dc.subjecttabletop Sweetenerspt_PT
dc.subjectQuantificationpt_PT
dc.subjectValidationpt_PT
dc.subjectDaily intakept_PT
dc.subjectComposição de Alimentospt_PT
dc.titleValidation of an HPLC-DAD/UV method for the quantification of cyclamate in tabletop sweeteners: risk of exceeding the acceptable daily intakept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage889pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage883pt_PT
oaire.citation.titleFood Additives and Contaminants: Part A: Chemistry, Analysis, Control, Exposure and Risk Assessmentpt_PT
oaire.citation.volume34pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Validation of HPLC method for quantification of cyclamate.pdf
Size:
610.29 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DAN - Artigos em revistas internacionais