Repository logo
 
Publication

Analysis Of The Cytotoxicity And Genotoxicity of Digested Titanium Dioxide Nanomaterials (TiO2) In Intestinal Cells

2020-09-12Conference object Restricted access
Simple item page
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorVieira, Adriana
dc.contributor.authorGramacho, Ana Catarina
dc.contributor.authorRolo, Dora
dc.contributor.authorVital, Nádia
dc.contributor.authorMartins, Carla
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorAlvito, Paula
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorBettencourt, Ana Francisca
dc.contributor.authorSilva, Maria João
dc.date.accessioned2020-10-28T16:33:42Z
dc.date.available2020-10-28T16:33:42Z
dc.date.issued2020-09-12
dc.description.abstractTitanium dioxide nanomaterials (TiO2) have been frequently applied as food additives, in pharmaceuticals and in personal care products, such as toothpastes. Despite some regulators like EFSA concluded that the absorption of orally administered TiO2 is low, and that the use of TiO2 as a food additive does not raise a genotoxic concern, the presence of TiO2 in human organs was recently reported. This exposure may lead to adverse outcomes and has been poorly investigated. Furthermore, many of the biological effects of TiO2 described in the literature often overlook adequate physicochemical properties and their modification due to NMs interaction with the surrounding physiological matrices happening, e.g, during digestion. This work aimed to investigate in intestinal cells, the cyto- and genotoxic effects of TiO2 after the simulation of the human digestive process using the standardized INFOGEST in vitro digestion method, to better understand their potential negative impacts on the gastrointestinal tract. The TiO2 were characterized before and after digestion using DLS, zeta potential and TEM-EDS. The digestion product was used for cytotoxicity (MTT) and genotoxicity (comet, micronucleus) assays in two types of intestinal cells (Caco-2 and mucus secreting HT29-MTX cells). The results of the cytotoxicity and genotoxicity assays are discussed in view of the TiO2 secondary characteristics, to further understand the potential adverse intestinal outcomes in light of the transformation they suffer during digestion.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through national funds (PIDDAC), PTDC/SAU-PUB/29481/2017 and co-funded by UIDB/00009/2020+UIDP/00009/2020 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT), iMed.ULisboa (PestUID/DTP/04138/2019) and CESAM (UID/AMB/50017/2019).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7226
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectIngested Nanomaterialspt_PT
dc.subjectNanotoxicologypt_PT
dc.subjectTitanium Dioxide Nanomaterialspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectToxicologiapt_PT
dc.titleAnalysis Of The Cytotoxicity And Genotoxicity of Digested Titanium Dioxide Nanomaterials (TiO2) In Intestinal Cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT (2019)
oaire.citation.conferencePlace(Virtual meeting)pt_PT
oaire.citation.titleEnvironmental Mutagenesis & Genomics Society Virtual Annual Meeting (EMGS), 12-16 September 2020pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameLouro
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication2361a951-8b9a-4b90-92d6-f6384003a242
relation.isAuthorOfPublication.latestForDiscovery2361a951-8b9a-4b90-92d6-f6384003a242
relation.isProjectOfPublication10a38358-808e-4126-a323-58973bebbcce
relation.isProjectOfPublicationf076fef9-580b-485a-9a0e-c5a599424c6e
relation.isProjectOfPublication.latestForDiscoveryf076fef9-580b-485a-9a0e-c5a599424c6e

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Louro 2020 EMGS poster.pdf
Size:
659.47 KB
Format:
Adobe Portable Document Format
Download

Collections

DGH - Posters/abstracts em congressos internacionais
DAN - Posters/abstracts em congressos internacionais