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Occupational exposure to formaldehyde and early biomarkers of cancer risk, immunotoxicity and susceptibility

2019-09-14Journal article Restricted access
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dc.contributor.authorCosta, Solange
dc.contributor.authorCosta, Carla
dc.contributor.authorMadureira, Joana
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorTeixeira-Gomes, Armanda
dc.contributor.authorGuedes de Pinho, Paula
dc.contributor.authorLaffon, Blanca
dc.contributor.authorTeixeira, João Paulo
dc.date.accessioned2020-05-08T11:32:03Z
dc.date.available2020-05-08T11:32:03Z
dc.date.issued2019-09-14
dc.description.abstractFormaldehyde (FA) is a high-volume production chemical manufactured worldwide to which many people are exposed to both environmentally and occupationally. FA was recently reclassified as a human carcinogen. Several epidemiological studies have revealed an increased risk of cancer development among workers exposed to FA. Although FA genotoxicity was confirmed in a variety of experimental systems, data from human studies are conflicting. The aim of the present study was to evaluate the occupational exposure to FA in a multistage approach relating the exposure with different biomarkers (dose and effect) and individual susceptibility. Air monitoring was performed to estimate the level of exposure to FA during shift work. Eighty-five workers from hospital anatomy-pathology laboratories exposed to FA and 87 controls were tested for cytogenetic alterations in lymphocytes (micronucleus, MN; sister-chromatid exchange, SCE) and T-cell receptor (TCR) mutation assay. The frequency of MN in exfoliated buccal cells, a first contact tissue was also assessed. Percentages of different lymphocyte subpopulations were selected as immunotoxicity biomarkers. The level of formic acid in urine was investigated as a potential biomarker of internal dose. The effects of polymorphic genes of xenobiotic metabolising enzymes and DNA repair enzymes on the endpoints studied were determined. The mean level of FA exposure was 0.38 ± 0.03 ppm. MN (in lymphocytes and buccal cells) and SCE were significantly increased in FA-exposed workers compared to controls. MN frequency positively correlated with FA levels of exposure and duration. Significant alterations in the percentage of T cytotoxic lymphocytes, NK cells and B lymphocytes were found between groups. Polymorphisms in CYP2E1, GSTP1 and FANCA genes were associated with increased genetic damage in FA-exposed subjects. The obtained information may provide new important data to be used by health and safety care programs and by governmental agencies responsible for setting the acceptable levels for occupational exposure to FA.pt_PT
dc.description.abstractHighlights: Micronucleus frequency (MN) positively correlated with formaldehyde levels of exposure and exposure duration; Strong association of MN levels between lymphocytes and buccal cells; Significant alterations in the percentage of T cytotoxic lymphocytes, NK cells and B lymphocytes; Polymorphisms on CYP2E1, GSTP1 and FANCA genes were associated with the effect endpoints levels.pt_PT
dc.description.sponsorshipSolange Costa, Carla Costa, Joana Madureira and Armanda Teixeira- Gomes are supported by Portuguese national funds, through Fundação para a Ciência e Tecnologia (FCT-MCTES), and by the European Social Fund, through Programa Operacional Capital Humano (POCH), respectively under the grants SFRH/BPD/100948/2014, SFRH/BPD/ 96196/2013, SFRH/BPD/115112/2016 and SFRH/BD/121802/2016. Vanessa Valdiglesias was supported by Xunta de Galicia (Spain) postdoctoral fellowship (reference ED481B 2016/190-0).
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEnviron Res. 2019 Dec;179(Pt A):108740. doi: 10.1016/j.envres.2019.108740. Epub 2019 Sep 14pt_PT
dc.identifier.doi10.1016/j.envres.2019.108740pt_PT
dc.identifier.issn0013-9351
dc.identifier.urihttp://hdl.handle.net/10400.18/6615
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Academic Presspt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0013935119305377?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAir Pollutants, Occupationalpt_PT
dc.subjectBiomarkerspt_PT
dc.subjectDNA Damagept_PT
dc.subjectFormaldehydept_PT
dc.subjectHumanspt_PT
dc.subjectLymphocytespt_PT
dc.subjectMicronucleus Testspt_PT
dc.subjectNeoplasmspt_PT
dc.subjectOccupational Exposurept_PT
dc.subjectMouth Mucosapt_PT
dc.subjectAr e Saúde Ocupacionalpt_PT
dc.titleOccupational exposure to formaldehyde and early biomarkers of cancer risk, immunotoxicity and susceptibilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issuePt Apt_PT
oaire.citation.startPage108740pt_PT
oaire.citation.titleEnvironmental Researchpt_PT
oaire.citation.volume179pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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