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How Hydroxyurea Alters the Gut Microbiome: A Longitudinal Study Involving Angolan Children with Sickle Cell Anemia

2022-08Journal article Open access
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dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorGinete, Catarina
dc.contributor.authorSantos, Brígida
dc.contributor.authorFernandes, Carolina
dc.contributor.authorSilva, Carina
dc.contributor.authorMiranda, Armandina
dc.contributor.authorVasconcelos, Jocelyne Neto de
dc.contributor.authorBrito, Miguel
dc.date.accessioned2023-02-08T15:42:25Z
dc.date.available2023-02-08T15:42:25Z
dc.date.issued2022-08
dc.descriptionThis article belongs to the Section Molecular Genetics and Genomics.pt_PT
dc.description.abstractSickle cell anemia (SCA) is an inherited hematological disorder and a serious global health problem, especially in Sub-Saharan Africa. Although hydroxyurea (HU) is the leading treatment for patients with SCA, its effects on the gut microbiome have not yet been explored. In this context, the aim of this study was to investigate this association by characterizing the gut microbiome of an Angolan SCA pediatric population before and after 6 months of HU treatment. A total of 66 stool samples were obtained and sequenced for the 16S rRNA gene (V3-V4 regions). Significant associations were observed in alpha and beta-diversity, with higher values of species richness for the children naïve for HU. We also noticed that children after HU had higher proportions of several beneficial bacteria, mostly short-chain fatty acids (SCFAs) producing species, such as Blautia luti, Roseburia inulinivorans, Eubacterium halli, Faecalibacterium, Ruminococcus, Lactobacillus rogosae, among others. In addition, before HU there was a higher abundance of Clostridium_g24, which includes C. bolteae and C. clostridioforme, both considered pathogenic. This study provides the first evidence of the HU effect on the gut microbiome and unravels several microorganisms that could be considered candidate biomarkers for disease severity and HU efficacy.pt_PT
dc.description.sponsorshipThis project was supported by FCT/Aga Khan (project No. 330842553), FCT/MCTES (UIDB/05608/2020 and UIDP/05608/2020)—H&TRC. The authors would like to acknowledge FCT for the financial support of the fellowships for M.D. (UI/BD/150705/2020) and C.G. (330842553/SCAFfoldChild/2020/BI/12 M).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Mol Sci. 2022 Aug 13;23(16):9061. doi: 10.3390/ijms23169061pt_PT
dc.identifier.doi10.3390/ijms23169061pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.18/8515
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationHealth and Technology Research Center
dc.relationHealth and Technology Research Center
dc.relationGut Microbiome Analysis in Patients with Sickle Cell Disease
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/16/9061pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHydroxyureapt_PT
dc.subjectGut Microbiomept_PT
dc.subjectSickle Cell Anemiapt_PT
dc.subjectAngolan Childrenpt_PT
dc.subject16S rRNApt_PT
dc.subjectPatologias do Glóbulo Vermelhopt_PT
dc.subjectAngola
dc.titleHow Hydroxyurea Alters the Gut Microbiome: A Longitudinal Study Involving Angolan Children with Sickle Cell Anemiapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleGut Microbiome Analysis in Patients with Sickle Cell Disease
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/UI%2FBD%2F150705%2F2020/PT
oaire.citation.issue16pt_PT
oaire.citation.startPage9061pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication875a9c35-558d-4af9-abf0-254eadf9819d
relation.isProjectOfPublicationc9a7f44a-b944-4ea0-a930-85b8e0b1bf1d
relation.isProjectOfPublication1317b5f3-f77d-4e28-9a47-40d11d6fc905
relation.isProjectOfPublication.latestForDiscovery875a9c35-558d-4af9-abf0-254eadf9819d

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