Publication
Ginkgo biloba L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage
| dc.contributor.author | Silva, Amélia | |
| dc.contributor.author | Silva, Sandra | |
| dc.contributor.author | Soares, Jorge | |
| dc.contributor.author | Martins-Gomes, Carlos | |
| dc.contributor.author | Teixeira, João Paulo | |
| dc.contributor.author | Leal, Fernanda | |
| dc.contributor.author | Gaivão, Isabel | |
| dc.date.accessioned | 2020-04-25T10:21:15Z | |
| dc.date.available | 2020-04-25T10:21:15Z | |
| dc.date.issued | 2019-11-29 | |
| dc.description.abstract | Ginkgo biloba L. leaf extracts and herbal infusions are used worldwide due to the health benefits that are attributed to its use, including anti-neoplastic, anti-aging, neuro-protection, antioxidant and others. The aim of this study was to evaluate the effect of an aqueous Ginkgo biloba extract on HepG2 cell viability, genotoxicity and DNA protection against paraquat-induced oxidative damage. Exposure to paraquat (PQ), over 24 h incubation at 1.0 and 1.5 µM, did not significantly reduce cell viability but induced concentration and time-dependent oxidative DNA damage. Ginkgo biloba leaf extract produced dose-dependent cytotoxicity (IC50 = 540.8 ± 40.5 µg/mL at 24 h exposure), and short incubations (1 h) produced basal and oxidative DNA damage (>750 and 1500 µg/mL, respectively). However, lower concentrations (e.g., 75 µg/mL) of Ginkgo biloba leaf extract were not cytotoxic and reduced basal DNA damage, indicating a protective effect at incubations up to 4 h. On the other hand, longer incubations (24 h) induced oxidative DNA damage. Co-incubation of HepG2 cells for 4 h, with G. biloba leaf extract (75 µg/mL) and PQ (1.0 or 1.5 µM) significantly reduced PQ-induced oxidative DNA damage. In conclusion, the consumption of Ginkgo biloba leaf extract for long periods at high doses/concentrations is potentially toxic; however, low doses protect the cells against basal oxidative damage and against environmentally derived toxicants that induce oxidative DNA damage. | pt_PT |
| dc.description.sponsorship | Financial support was received from the INTERACT project—“Integrative Research in Environment, Agro-Chains and Technology”, no. NORTE-01-0145-FEDER-000017, in its line of research entitled ISAC, co-financed by the European Regional Development Fund (ERDF) through NORTE 2020 (North Regional Operational Program 2014/2020). C.M.-G. was supported by a grant from INTERACT (BIM/UTAD/30/2018). The Portuguese Science and Technology Foundation (FCT) is also acknowledged under the projects UID/AGR/04033/2019 (CITAB) and UID/CVT/00772/2019 (CECAV). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Plants (Basel). 2019 Nov 29;8(12):556. doi: 10.3390/plants8120556 | pt_PT |
| dc.identifier.doi | 10.3390/plants8120556 | pt_PT |
| dc.identifier.issn | 2223-7747 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6515 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation.publisherversion | https://www.mdpi.com/2223-7747/8/12/556 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Ginkgo biloba L | pt_PT |
| dc.subject | Aqueous Leaf Extract | pt_PT |
| dc.subject | Comet Assay | pt_PT |
| dc.subject | Genotoxicity | pt_PT |
| dc.subject | HepG2 Cells | pt_PT |
| dc.subject | Paraquat | pt_PT |
| dc.subject | Genotoxicidade Ambiental | pt_PT |
| dc.title | Ginkgo biloba L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F04033%2F2013/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00772%2F2013/PT | |
| oaire.citation.issue | 12 | pt_PT |
| oaire.citation.startPage | 556 | pt_PT |
| oaire.citation.title | Plants | pt_PT |
| oaire.citation.volume | 8 | pt_PT |
| oaire.fundingStream | 5876 | |
| oaire.fundingStream | 5876 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | 2854d17f-1c22-46c6-a66d-f03b7bbd0011 | |
| relation.isProjectOfPublication | 5b4d6801-0afc-469c-be6c-ebc7252bb4d2 | |
| relation.isProjectOfPublication.latestForDiscovery | 5b4d6801-0afc-469c-be6c-ebc7252bb4d2 |