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Publicação
Fungal burden exposure assessment in podiatry clinics from Ireland
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Orientador(es)
Resumo(s)
Fungi are amongst the bioaerosols of most importance, as indicated by
the growing interest in this field of research. The aim was to characterize
the exposure to fungal burden in podiatry clinics using culture-based and
molecular methods. Methods: Airborne fungi were collected using an
impaction air sampler and surface samples were also performed. Fourteen air
samples were collected for direct detection of fungal DNA from filamentous
fungi and dermatophytes. Overall, 63.6 % of the evening samples and
46 % of the morning samples surpassed the threshold values (150 CFU/
m3). Molecular detection, by real time PCR, of the target fungal species/
strains (Aspergillus and Stachybotrys species) was negative for all samples
collected. Trichophyton rubrum was detected by PCR analysis in one DNA
sample collected on day six. Results suggest the use of both culture-based
and molecular methodologies are desirable for a complete evaluation of
fungal burden in this particular health care setting.
Descrição
Palavras-chave
Podiatry Fungal Exposure Dermatophytes Environmental Samples PCR Infecções Sistémicas e Zoonoses
Contexto Educativo
Citação
Int J Environ Health Res. 2018 Apr;28(2):167-177. doi: 10.1080/09603123.2018.1453053. Epub 2018 Mar 26
Editora
Taylor & Francis