Publication
Microcystin-LR activates the ERK1/2 kinases and stimulates the proliferation of the monkey kidney-derived cell line Vero-E6
| dc.contributor.author | Dias, E. | |
| dc.contributor.author | Matos, P. | |
| dc.contributor.author | Pereira, P. | |
| dc.contributor.author | Batoréu, M.C.C. | |
| dc.contributor.author | Silva, M.J. | |
| dc.contributor.author | Jordan, P. | |
| dc.date.accessioned | 2011-09-06T15:50:15Z | |
| dc.date.available | 2011-09-06T15:50:15Z | |
| dc.date.issued | 2010-09 | |
| dc.description.abstract | Microcystin-LR (MCLR) is a peptide produced by freshwater cyanobacteria that induces severe hepatotoxicity in humans and animals. MCLR is also a potent tumour promoter and it has been proposed that this activity is mediated by the inhibition of protein phosphatases PP1/PP2A, possibly through the activation of proto-oncogenes c-jun, c-fos and c-myc. However, the mechanisms underlying MCLR-induced tumour promotion are still largely unknown, particularly in non-liver cells. In previous studies we have demonstrated that micromolar concentrations of MCLR induce cytotoxic effects in the kidney Vero-E6 cell line. The purpose of the present work was to evaluate whether the exposure to subcytotoxic concentrations of MCLR was sufficient to induce the proliferation of Vero-E6 cells. Through BrdU incorporation assay we show that at nanomolar concentrations MCLR stimulates cell cycle progression in Vero-E6 kidney cell line. Moreover, the analysis of mitogen-activated protein kinases p38, JNK and ERK1/2 activity revealed that the proliferative effect of MCLR is associated with the activation of the pro-proliferative ERK1/2 pathway. These results emphasise the importance to confirm in vivo the impact of MCLR on tumour promotion at kidney level. | por |
| dc.identifier.citation | Toxicol In Vitro. 2010 Sep;24(6):1689-95. Epub 2010 Jun 1 | por |
| dc.identifier.issn | 0887-2333 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/120 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S088723331000127X | |
| dc.subject | Microcystin-LR | por |
| dc.subject | Tumour promotion | por |
| dc.subject | ERK1/2 | por |
| dc.subject | Kidney | por |
| dc.subject | Vias de Transdução de Sinal e Patologias Associadas | por |
| dc.subject | Toxicologia | por |
| dc.title | Microcystin-LR activates the ERK1/2 kinases and stimulates the proliferation of the monkey kidney-derived cell line Vero-E6 | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1695 | por |
| oaire.citation.startPage | 1689 | por |
| oaire.citation.title | Toxicology in Vitro | por |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |