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Human hepatoma cells exposed to estuarine sediment contaminant extracts permitted the differentiation between cytotoxic and pro-mutagenic fractions

2013-11-22Journal article Restricted access
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dc.contributor.authorPinto, Miguel
dc.contributor.authorCosta, Pedro Manuel
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorCosta, Maria Helena
dc.contributor.authorLavinha, João
dc.contributor.authorCaeiro, Sandra
dc.contributor.authorSilva, Maria João
dc.date.accessioned2014-03-07T17:35:58Z
dc.date.available2014-03-07T17:35:58Z
dc.date.issued2013-11-22
dc.description.abstractComplex toxicant mixtures present in estuarine sediments often render contaminant screening unfeasible and compromise determining causation. HepG2 cells were subjected to bioassays with sediment extracts obtained with a series of progressively polar solvents plus a crude extract. The sediments were collected from an impacted area of an estuary otherwise regarded as pristine, whose stressors result mostly from aquaculture effluents and hydrodynamic shifts that enhance particle deposition. Compared to a reference scenario, the most polar extracts yielded highest cytotoxicity while higher genotoxicity (including oxidative damage) was elicited by non-polar solvents. While the former caused effects similar to those expected from biocides, the latter triggered effects compatible with known pro-mutagens like PAHs, even though the overall levels of toxicants were considered of low risk. The results indicate that the approach may constitute an effective line-of-evidence to infer on the predominant set of hazardous contaminants present in complex environmental mixtures.por
dc.identifier.citationEnviron Pollut. 2014;185:141-8. Epub 2013 Nov 24.por
dc.identifier.issn0269-7491
dc.identifier.issndoi: 10.1016/j.envpol.2013.10.034
dc.identifier.urihttp://hdl.handle.net/10400.18/1971
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationFCT and co-financed by the European Community FEDER through the program COMPETE project reference PTDC/SAU-ESA/100107/2008por
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0269749113005629por
dc.subjectGenotoxicidade Ambientalpor
dc.subjectEstuarine Sedimentspor
dc.subjectHepG2 Cellspor
dc.subjectComet Assaypor
dc.subjectGenotoxicitypor
dc.subjectCytotoxicitypor
dc.subjectSediment contaminationpor
dc.subjectSolvent extractionpor
dc.subjectMira Estuarypor
dc.titleHuman hepatoma cells exposed to estuarine sediment contaminant extracts permitted the differentiation between cytotoxic and pro-mutagenic fractionspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage148por
oaire.citation.startPage141por
oaire.citation.titleEnvironmental Pollutionpor
oaire.citation.volume185Cpor
rcaap.rightsembargoedAccesspor
rcaap.typearticlepor

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