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Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants

2018-08-27Journal article Restricted access
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dc.contributor.authorFabre, Adrien
dc.contributor.authorOleastro, Monica
dc.contributor.authorNunes, Alexandra
dc.contributor.authorSantos, Andrea
dc.contributor.authorSifré, Elodie
dc.contributor.authorDucournau, Astrid
dc.contributor.authorBénéjat, Lucie
dc.contributor.authorBuissonnière, Alice
dc.contributor.authorFloch, Pauline
dc.contributor.authorMégraud, Francis
dc.contributor.authorDubois, Véronique
dc.contributor.authorLehours, Philippe
dc.date.accessioned2019-03-20T18:22:07Z
dc.date.available2019-03-20T18:22:07Z
dc.date.issued2018-08-27
dc.descriptionFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113490/pt_PT
dc.description.abstractA (WGS) approach was conducted in order to identify the molecular determinants associated with antimicrobial resistance in 12 multidrug-resistant Campylobacter jejuni and Campylobacter coli isolates, with a focus on aminoglycoside resistance determinants. Two variants of a new aminoglycoside phosphotransferase gene [aph(2″)-Ii1 and aph(2″)-Ii2 ] putatively associated with gentamicin resistance were found. In addition, the following new genes were identified for the first time in Campylobacter: a lincosamide nucleotidyltransferase gene [lnu(G)], likely associated with lincomycin resistance, and two resistance enzyme genes (spw and apmA) similar to those found in Staphylococcus aureus, which may confer spectinomycin and gentamicin resistance, respectively. A C1192T mutation of the 16S rRNA gene that may be involved in spectinomycin resistance was also found in a C. coli isolate. Genes identified in the present study were located either on the bacterial chromosome or on plasmids that could be transferred naturally. Their role in aminoglycoside resistance remains to be supported by genetic studies. Regarding the other antimicrobial agents studied, i.e., ampicillin, ciprofloxacin, erythromycin, and tetracycline, a perfect correlation between antimicrobial phenotypes and genotypes was found. Overall, our data suggest that WGS analysis is a powerful tool for identifying resistance determinants in Campylobacter and can disclose the full genetic elements associated with resistance, including antimicrobial compounds not tested routinely in antimicrobial susceptibility testing.pt_PT
dc.description.sponsorshipThis work was supported by internal funding of the Ricardo Jorge National Institute of Health. WGS was performed at the Unidade de Tecnologia e Inovação (Departamento de Genética Humana, Instituto Nacional de Saúde Ricardo Jorge, Lisbon, Portugal).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Clin Microbiol. 2018 Aug 27;56(9). pii: e00390-18. doi: 10.1128/JCM.00390-18pt_PT
dc.identifier.doi10.1128/JCM.00390-18pt_PT
dc.identifier.issn0095-1137
dc.identifier.urihttp://hdl.handle.net/10400.18/6257
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relation.publisherversionhttps://jcm.asm.org/content/56/9/e00390-18.longpt_PT
dc.subjectCampylobacterpt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectAminoglycoside Resistancept_PT
dc.subjectAminoglycoside-modifying Enzymespt_PT
dc.subjectMultidrug Resistancept_PT
dc.subjectWhole-genome Sequencingpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleWhole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinantspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.startPagepii: e00390-18pt_PT
oaire.citation.titleJournal of Clinical Microbiologypt_PT
oaire.citation.volume56pt_PT
rcaap.embargofctPolítica editorial a revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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