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Cytotoxicity and genotoxicity of MWCNT-7 and crocidolite: assessment in alveolar epithelial cells versus their coculture with monocyte-derived macrophages

2020-05Journal article Restricted access
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dc.contributor.authorVentura, Célia
dc.contributor.authorPereira, Joana F.S.
dc.contributor.authorMatos, Paulo
dc.contributor.authorMarques, Bárbara
dc.contributor.authorJordan, Peter
dc.contributor.authorSousa-Uva, António
dc.contributor.authorSilva, Maria João
dc.date.accessioned2021-03-06T15:14:26Z
dc.date.available2021-03-06T15:14:26Z
dc.date.issued2020-05
dc.description.abstractIn the past years, several in vitro studies have addressed the pulmonary toxicity of multi-walled carbon nanotubes (MWCNT) and compared it with that caused by asbestos fibers, but their conclusions have been somewhat inconsistent and difficult to extrapolate to in vivo. Since cell coculture models were proposed to better represent the in vivo conditions than conventional monocultures, this work intended to compare the cytotoxicity and genotoxicity of MWCNT-7 (Mitsui-7) and crocidolite using A549 cells grown in a conventional monoculture or in coculture with THP-1 macrophages. Although a decrease in A549 viability was noted following exposure to a concentration range of MWCNT-7 and crocidolite, no viability change occurred in similarly exposed cocultures. Early events indicating epithelial to mesenchymal transition (EMT) were observed which could explain apoptosis resistance. The comet assay results were similar between the two models, being positive and negative for crocidolite and MWCNT-7, respectively. An increase in the micronucleus frequency was detected in the cocultured A549-treated cells with both materials, but not in the monoculture. On the other hand, exposure of A549 monocultures to MWCNT-7 induced a highly significant increase in nucleoplasmic bridges in which those were found embedded. Our overall results demonstrate that (i) both materials are cytotoxic and genotoxic, (ii) the presence of THP-1 macrophages upholds the viability of A549 cells and increases the aneugenic/clastogenic effects of both materials probably through EMT, and (iii) MWCNT-7 induces the formation of nucleoplasmic bridges in A549 cells.pt_PT
dc.description.sponsorshipThis research was supported by the Foundation for Science and Technology, Center for Toxicogenomics and Human Health (ToxOmics) under grant (UID/BIM/00009/2013) and by ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017) through national funds (PIDDAC).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanotoxicology. 2020 May;14(4):479-503. doi: 10.1080/17435390.2019.1695975. Epub 2020 Feb 12.pt_PT
dc.identifier.doi10.1080/17435390.2019.1695975pt_PT
dc.identifier.issn1743-5390
dc.identifier.urihttp://hdl.handle.net/10400.18/7341
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor and Francispt_PT
dc.relation.publisherversionhttps://www.tandfonline.com/doi/abs/10.1080/17435390.2019.1695975?journalCode=inan20pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAlveolar Epithelial Cellspt_PT
dc.subjectAsbestos, Crocidolitept_PT
dc.subjectCell Survivalpt_PT
dc.subjectCoculture Techniquespt_PT
dc.subjectComet Assaypt_PT
dc.subjectEpithelial-Mesenchymal Transitionpt_PT
dc.subjectHumanspt_PT
dc.subjectMacrophagespt_PT
dc.subjectNanotubes, Carbonpt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectVias de Transdução de Sinal e Patologias Associadaspt_PT
dc.titleCytotoxicity and genotoxicity of MWCNT-7 and crocidolite: assessment in alveolar epithelial cells versus their coculture with monocyte-derived macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.citation.endPage503pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage479pt_PT
oaire.citation.titleNanotoxicologypt_PT
oaire.citation.volume14pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione9cc9728-4f09-4e3a-b30d-53d4429986fb
relation.isProjectOfPublication.latestForDiscoverye9cc9728-4f09-4e3a-b30d-53d4429986fb

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