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LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients

2019-11-27Journal article Restricted access
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dc.contributor.authorOuhib, Salma
dc.contributor.authorVidal, Arnau
dc.contributor.authorMartins, Carla
dc.contributor.authorGali, Ridha
dc.contributor.authorHedhili, Abderrazzeki
dc.contributor.authorDe Saeger, Sarah
dc.contributor.authorDe Boevre, Marthe
dc.date.accessioned2020-04-27T18:33:09Z
dc.date.available2020-04-27T18:33:09Z
dc.date.issued2019-11-27
dc.description.abstractBiomarker-driven research has been proposed as a successful method to assess the exposure of individuals to xenobiotics, including mycotoxins, through estimation of their metabolites in biological fluids. A methodology to determine patulin (PAT) and citrinin (CIT) in human urine and plasma using liquid chromatography coupled to tandem mass spectrometry was developed and validated in the present study. Selectivity/specificity, linearity, limit of detection and quantification, apparent recovery, intraday- and interday-precision and measurement uncertainty were investigated for validation purposes. Finally, the method was used to analyze human urine (n = 100) and plasma (n = 100) case-control samples, where 50 samples originated from colorectal cancer patients and 50 from age/sex-matched controls. This case-control study revealed that PAT was not detected in urine samples, however occurred in 25% of the analysed plasma samples with an average concentration of 11.62 ± 6.67 ng/mL in the positive samples. CIT was found in urine samples (74%) and plasma samples (36%) with average concentrations in the positive samples of 0.45 ± 0.24 ng/mL and 0.49 ± 0.2 ng/mL respectively. No statistically significant difference of PAT and CIT concentration among colorectal cancer and control patients (p > 0.05) was observed.pt_PT
dc.description.abstractHighlights: Development and validation of an LC-MS/MS method to determine patulin and citrinin in biological fluids; Patulin was detected in plasma (25%) for the first time; Citrinin was present in 74% of Tunisian urine samples; Citrinin was present in 36% of Tunisian plasma samples.pt_PT
dc.description.sponsorshipThe authors acknowledge the support of the Tunisian Ministry of Education and Scientific Research, the Toxicology and Environment Research Laboratory LR12SP07 (CAMUR, Tunis, Tunisia) and MYTOXSOUTH (https://mytoxsouth.org) (Ghent University and Global Minds International Thematic Network). Dr. Arnau Vidal expresses thanks to The Research Foundation Flanders(Wetenschappelijk Oderzoek- Vlaanderen, FWO) for the postdoctoral grant (12Y1719N).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Chem Toxicol. 2020 Feb;136:110994. doi: 10.1016/j.fct.2019.110994. Epub 2019 Nov 27pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.fct.2019.110994pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10400.18/6533
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Pergamonpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0278691519307847?via%3Dihubpt_PT
dc.subjectLC-MS/MSpt_PT
dc.subjectMycotoxin Exposurept_PT
dc.subjectCancerpt_PT
dc.subjectPlasmapt_PT
dc.subjectUrinept_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectToxicologiapt_PT
dc.subjectAvaliação de Riscopt_PT
dc.subjectSaúde Humanapt_PT
dc.titleLC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patientspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage110994pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume136pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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