Publication
Genotypic and phenotypic features of Arcobacter butzleri pathogenicity
| dc.contributor.author | Ferreira, S. | |
| dc.contributor.author | Queiroz, J.A. | |
| dc.contributor.author | Oleastro, M. | |
| dc.contributor.author | Domingues, F.C. | |
| dc.date.accessioned | 2015-02-19T15:08:22Z | |
| dc.date.available | 2015-02-19T15:08:22Z | |
| dc.date.issued | 2014-11 | |
| dc.description.abstract | Even though Arcobacter butzleri has been implicated in some human disease as diarrhoea and bacteraemia, much of its pathogenesis and virulence factors remain unclear. In this work we have compared pathogenic and genotypic properties of six A. butzleri isolates from human and non-human sources. The tested isolates showed to be susceptible to tetracyclines and aminoglycosides, however non-human isolates were all resistant to quinolones. The ability to form biofilms was variable among the tested strains, and all of them showed a weak haemolytic activity. The presence of nine putative virulence genes was determined, with cadF, ciaB, cj1349, mviN, pldA, tlyA being detected in all strains, while irgA (3/6), hecA (5/6), hecB (4/6) were detected only in some strains. High levels of adhesion were observed for A. butzleri on Caco-2 cells, with pre-existing inflammation showing no significant effect on the adherence ability; yet variable levels of invasion were observed. A. butzleri isolates were able to survive intracellularly in Caco-2 cells and to induce a significant up-regulation of interleukin-8 secretion and structural cell rearrangements. These data brings new insights on A. butzleri virulence and highlights its pathogenic potential. | por |
| dc.description.sponsorship | This work was partially supported by the project PEst-C/SAU/UI0709/2011 funded by Fundação para a Ciência e a Tecnologia through the program COMPETE. | por |
| dc.identifier.citation | Microb Pathog. 2014 Nov;76:19-25. doi: 10.1016/j.micpath.2014.09.004. Epub 2014 Sep 10 | por |
| dc.identifier.doi | 10.1016/j.micpath.2014.09.004 | |
| dc.identifier.issn | 0882-4010 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/2925 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier/ Academic Press | por |
| dc.relation | SFRH/BD/66857/2009 from FCT | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0882401014001284 | por |
| dc.subject | Adhesion | por |
| dc.subject | Arcobacter Butzleri | por |
| dc.subject | Inflammation | por |
| dc.subject | Intracellular Survival | por |
| dc.subject | Invasion | por |
| dc.subject | Virulence | por |
| dc.subject | Infecções Gastrointestinais | por |
| dc.title | Genotypic and phenotypic features of Arcobacter butzleri pathogenicity | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 25 | por |
| oaire.citation.startPage | 19 | por |
| oaire.citation.title | Microbial Pathogenesis | por |
| oaire.citation.volume | 76 | por |
| rcaap.rights | embargoedAccess | por |
| rcaap.type | article | por |
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