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Capsular Type, Sequence Type and Microbial Resistance Factors Impact on DNase Activity of Streptococcus agalactiae Strains from Human and Bovine Origin

2018-12-11Journal article Open access
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dc.contributor.authorFlorindo, Carlos
dc.contributor.authorBarroco, Cinthia Alves
dc.contributor.authorSilvestre, Inês
dc.contributor.authorDamião, Vera
dc.contributor.authorGomes, João Paulo
dc.contributor.authorSpellerberg, Barbara
dc.contributor.authorSantos-Sanches, Ilda
dc.contributor.authorBorrego, Maria José
dc.date.accessioned2019-03-22T15:37:27Z
dc.date.available2019-03-22T15:37:27Z
dc.date.issued2018-12-11
dc.description.abstractExtracellular deoxyribonucleases (DNases) contribute to the spread of pathogenic bacteria through the evasion from host innate immunity. Our main objective was to evaluate the production of extracellular DNases by human and bovine Streptococcus agalactiae clinical strains and perform a correlation of genetic lineages and DNase activity with capsular type, genetic determinants, clinical origin (colonization and infection), and host (human or bovine). DNase activity was evaluated by qualitative and quantitative assays for a collection of 406 human (n = 285) and bovine (n = 121) strains. All (121/121) bovine were isolated from mastitis and revealed to be DNase (+), indicating a putative pathogenic role in this clinical scenario. From the human S. agalactiae strains, 86% (245/285) showed DNase activity, among which all strains belonging to capsular types, namely, Ia, Ib, III-2, and IV. All CC17 strains (n = 58) and 56/96 (58.3%) of the CC19 displayed DNase activity. DNase (-) strains belonged to the CC19 group. However, the subcharacterization of CC19 S. agalactiae strains through multiple-locus variable number tandem repeat analysis (MLVA), antibiotic resistance, mobile elements, and surface proteins did not provide any distinction among DNase producers and non-producers. The production of DNases by all human CC17 strains, about two-fifths of human CC19, and all bovine strains, suggest an important contribution of DNases to hypervirulence.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e Tecnologia, Portugal [grant numbers PTDC/SAU-MII/105114/ 2008, PTDC/CVT-EPI/6685/2014, SFRH/BD/48231/2008, and SFRH/BD/118350/2016].pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEur J Microbiol Immunol (Bp). 2018 Dec 11;8(4):149-154. doi: 10.1556/1886.2018.00026. eCollection 2018 Dec 23pt_PT
dc.identifier.doi10.1556/1886.2018.00026pt_PT
dc.identifier.issn2062-509X
dc.identifier.urihttp://hdl.handle.net/10400.18/6277
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAkadémiai Kiadópt_PT
dc.relation.publisherversionhttps://akademiai.com/doi/10.1556/1886.2018.00026pt_PT
dc.subjectDNasespt_PT
dc.subjectStreptococcus agalactiaept_PT
dc.subjectGBSpt_PT
dc.subjectCC17pt_PT
dc.subjectCC19pt_PT
dc.subjectDNase Activity Assayspt_PT
dc.subjectBovine and Human Strainspt_PT
dc.subjectExtracellular DNasespt_PT
dc.subjectInfecções Sexualmente Transmissíveispt_PT
dc.titleCapsular Type, Sequence Type and Microbial Resistance Factors Impact on DNase Activity of Streptococcus agalactiae Strains from Human and Bovine Originpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MII%2F105114%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F48231%2F2008/PT
oaire.citation.endPage154pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage149pt_PT
oaire.citation.titleEuropean Journal of Microbiology and Immunologypt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication46fd75b3-6192-4106-b2fc-b0af9afddb51
relation.isProjectOfPublicationd7990da8-e7cc-46a4-9e62-3f856c1fb88c
relation.isProjectOfPublication.latestForDiscovery46fd75b3-6192-4106-b2fc-b0af9afddb51

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