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Characterization of Haemophilus influenzae from healthy children attending day-care centers in the Lisbon regiĆ³n, 2015-2019

2021-07-09Conference object Restricted access
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dc.contributor.authorBajanca Lavado, Maria Paula
dc.contributor.authorCavaco, LuĆ­s Filipe
dc.contributor.authorFernandes, Mariana
dc.contributor.authorTouret, Tiago
dc.contributor.authorCandeias, Catarina
dc.date.accessioned2022-07-08T08:58:59Z
dc.date.available2023-01-01T01:30:28Z
dc.date.issued2021-07-09
dc.description.abstractHaemophilus influenzae colonizes the human upper respiratory tract, where it can remain asymptomatically. It can also progress from colonizer to pathogen and cause mucosal or invasive infections. The aim of this study was to unravel epidemiological aspects of H. influenzae nasopharyngeal colonization in healthy children attending day-care centers in Portugal. Methods Between 2015 and 2019, 1518 nasopharyngeal samples were collected from children up to six years old, attending day-care centers in Lisbon region. Samples were cultured in chocolate agar with bacitracin and isovitalex and screened for the presence of H. influenzae based on colony morphology. Pure cultures were obtained and capsular serotype was determined by PCR. Ī²-lactamase production was assessed with nitrocefin. Antibiotic susceptibility was determined for all Ī²-lactamase producer isolates and all encapsulated isolates by a microdilution assay. Genetic characterization based on whole genome sequencing (WGS) analysis was performed for encapsulated isolates. Results H. influenzae was presumptively identified in 1280 samples indicating a high carriage rate (84.3%). Of these, most isolates (96.7%) were non-encapsulated (NT). The 42 encapsulated isolates belonged to serotypes a (n=4), b (n=1), e (n=14), and f (n=23). A total of 7.5% (n=96) of the isolates were Ī²-lactamase producers although a higher percentage (11.9%) was observed in encapsulated isolates. Most isolates were susceptible to all antimicrobials tested. MLST revealed low genetic variability among encapsulated isolates: Hia-ST23, Hib-ST6, Hie-ST18 and ST122; and Hif-ST124, ST973 and ST2346. Analysis of presence/absence of 105 virulence genes showed that this is associated with the serotype, with serotype b isolates having the highest number of virulence genes. Virulence genes associated with specific structures or functions, such as iron acquisition, adherence, biofilm development or immune evasion, were present in all isolates. Conclusion Our results show that, in children attending day-care centers in the Lisbon region, the proportion of H. influenzae carriers is high and that circulation of encapsulated isolates is rare. Characterization of circulating isolates is important for community surveillance as these isolates may progress to cause severe invasive disease.pt_PT
dc.description.sponsorshipThis work was financially supported by projects LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020 ā€“ Programa Operacional Competitividade e InternacionalizaĆ§Ć£o) and LISBOA-01-0145-FEDER-016417 (ONEIDA co-funded by Fundos Europeus Estruturais e de Investimento, Programa Operacional Regional Lisboa 2020 and FundaĆ§Ć£o para a CiĆŖncia e a Tecnologia (FCT)) and Department of Infectious Diseases of National Institute of Health, Lisbon Portugal.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.citationMP Bajanca-Lavado, L.F: Cavaco, M. Fernandes T. Touret, Candeias C, R. SĆ”-LeĆ£o. Characterization of Haemophilus influenzae from healthy children attending day-care centers in the Lisbon regiĆ³n, 2015-2019. Poster (03711) apresentado no 31st ECCMIDpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8091
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectHaemophilus influenzaept_PT
dc.subjectH. influenzae Colonizationpt_PT
dc.subjectChildrenpt_PT
dc.subjectSerotypespt_PT
dc.subjectAntibiotic Resistencept_PT
dc.subjectWhole Genome Sequencingpt_PT
dc.subjectInfecƧƵes RespiratĆ³riaspt_PT
dc.subjectResistĆŖncia aos Antimicrobianospt_PT
dc.titleCharacterization of Haemophilus influenzae from healthy children attending day-care centers in the Lisbon regiĆ³n, 2015-2019pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.title31st European Congress on Clinical Microbiology and Infectious Diseases, 9-12 July 2021pt_PT
person.familyNameBajanca Lavado
person.givenNameMaria Paula
person.identifier.ciencia-id2B11-E245-3D39
person.identifier.orcid0000-0002-1453-0315
person.identifier.scopus-author-id6506614938
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication31093b71-e0a5-4796-8b01-12f4eccfc57a
relation.isAuthorOfPublication.latestForDiscovery31093b71-e0a5-4796-8b01-12f4eccfc57a

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