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Tumour-like phenotypes in urothelial cells after exposure to antigens from eggs of Schistosoma haematobium: an oestrogen-DNA adducts mediated pathway?

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dc.contributor.authorBotelho, Mónica C.
dc.contributor.authorVale, N.
dc.contributor.authorGouveia, M.J.
dc.contributor.authorRinaldi, G.
dc.contributor.authorSantos, J.
dc.contributor.authorSantos, L.L.
dc.contributor.authorGomes, P.
dc.contributor.authorBrindley, P.J.
dc.contributor.authorCorreia da Costa, José Manuel
dc.date.accessioned2013-01-28T12:02:06Z
dc.date.available2013-01-28T12:02:06Z
dc.date.issued2013-01
dc.description.abstractChronic infection with the blood fluke, Schistosoma haematobium, is associated with squamous cell carcinoma of the bladder. Previously, it has been shown that soluble extracts of mixed sex adult S. haematobium worms (SWAP) are tumourigenic, both in vitro and in vivo. In addition, oestrogen-related molecules in SWAP of S. haematobium down-regulate oestrogen receptors (ERs) alpha and beta in oestrogen responsive cells. Moreover, schistosome oestrogens occur in sera of persons with schistosomiasis haematobia and repress transcription of ERs in urothelial cells. Given that eggs of S. haematobium are the developmental stage directly responsible for urogenital disease during schistosomiasis haematobia, we suspected that soluble antigens from S. haematobium eggs exhibit similar or more potent tumorigenic capacity. Here we investigated the tumorigenic potential of soluble egg antigens (Sh-SEA) of S. haematobium and the endocrine system in favouring parasitism by schistosomes. The findings confirmed that 6.25μg/ml of Sh-SEA was enough to stimulate cell proliferation, reduce apoptosis and increase oxidative stress of Sh-SEA-exposed urothelial cells. In addition, genotoxic effects of Sh-SEA on these cells were determined by using alkaline single-cell gel electrophoresis (Comet). Furthermore, Liquid Chromatography Diode Array Detection Electron Spray Ionisation Mass Spectrometry indicated the presence of catechol-oestrogens in S. haematobium SEA. A prospective oestrogen-DNA adduct mediated pathway in S. haematobium egg induced bladder cancer is also discussed.por
dc.identifier.citationInt J Parasitol. 2013 Jan;43(1):17-26. Epub 2012 Dec 20.por
dc.identifier.issn0020-7519
dc.identifier.otherdoi: 10.1016/j.ijpara.2012.10.023
dc.identifier.urihttp://hdl.handle.net/10400.18/1165
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0020751912003104por
dc.subjectSchistosoma Haematobiumpor
dc.subjectEggspor
dc.subjectHCV29 Cellspor
dc.subjectOxidative Stresspor
dc.subjectGenotoxicitypor
dc.subjectOestrogen–DNA Adductspor
dc.subjectBladder Cancerpor
dc.subjectLC-DAD/ESI-MSpor
dc.titleTumour-like phenotypes in urothelial cells after exposure to antigens from eggs of Schistosoma haematobium: an oestrogen-DNA adducts mediated pathway?por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage26por
oaire.citation.startPage17por
oaire.citation.titleInternational Journal for Parasitologypor
oaire.citation.volume43(1)por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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