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Listeria monocytogenes from Food Products and Food Associated Environments: Antimicrobial Resistance, Genetic Clustering and Biofilm Insights

2024-05-14Journal article Open access
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dc.contributor.authorSilva, Adriana
dc.contributor.authorSilva, Vanessa
dc.contributor.authorGomes, João Paulo
dc.contributor.authorCoelho, Anabela
dc.contributor.authorBatista, Rita
dc.contributor.authorSaraiva, Cristina
dc.contributor.authorEsteves, Alexandra
dc.contributor.authorMartins, Ângela
dc.contributor.authorContente, Diogo
dc.contributor.authorDiaz-Formoso, Lara
dc.contributor.authorCintas, Luis M.
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorBorges, Vítor
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2025-02-19T15:55:10Z
dc.date.available2025-02-19T15:55:10Z
dc.date.issued2024-05-14
dc.description.abstractListeria monocytogenes, a foodborne pathogen, exhibits high adaptability to adverse environmental conditions and is common in the food industry, especially in ready-to-eat foods. L. monocytogenes strains pose food safety challenges due to their ability to form biofilms, increased resistance to disinfectants, and long-term persistence in the environment. The aim of this study was to evaluate the presence and genetic diversity of L. monocytogenes in food and related environmental products collected from 2014 to 2022 and assess antibiotic susceptibility and biofilm formation abilities. L. monocytogenes was identified in 13 out of the 227 (6%) of samples, 7 from food products (meat preparation, cheeses, and raw milk) and 6 from food-processing environments (slaughterhouse-floor and catering establishments). All isolates exhibited high biofilm-forming capacity and antibiotic susceptibility testing showed resistance to several classes of antibiotics, especially trimethoprim-sulfamethoxazole and erythromycin. Genotyping and core-genome clustering identified eight sequence types and a cluster of three very closely related ST3 isolates (all from food), suggesting a common contamination source. Whole-genome sequencing (WGS) analysis revealed resistance genes conferring resistance to fosfomycin (fosX), lincosamides (lin), fluoroquinolones (norB), and tetracycline (tetM). In addition, the qacJ gene was also detected, conferring resistance to disinfecting agents and antiseptics. Virulence gene profiling revealed the presence of 92 associated genes associated with pathogenicity, adherence, and persistence. These findings underscore the presence of L. monocytogenes strains in food products and food-associated environments, demonstrating a high virulence of these strains associated with resistance genes to antibiotics, but also to disinfectants and antiseptics. Moreover, they emphasize the need for continuous surveillance, effective risk assessment, and rigorous control measures to minimize the public health risks associated to severe infections, particularly listeriosis outbreaks. A better understanding of the complex dynamics of pathogens in food products and their associated environments can help improve overall food safety and develop more effective strategies to prevent severe health consequences and economic losses.pt_PT
dc.description.sponsorshipThis work was supported by the projects UIDB/00772/2020 (Doi:10.54499/UIDB/00772/2020), funded by the Portuguese Foundation for Science and Technology (FCT).
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntibiotics (Basel). 2024 May 14;13(5):447. doi: 10.3390/antibiotics13050447
dc.identifier.doi10.3390/antibiotics13050447pt_PT
dc.identifier.issn2079-6382
dc.identifier.pmid38786175
dc.identifier.urihttp://hdl.handle.net/10400.18/10371
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPI
dc.relationVeterinary and Animal Science Research Centre
dc.relation.hasversionhttps://www.mdpi.com/2079-6382/13/5/447
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/13/5/447pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectListeria monocytogenespt_PT
dc.subjectFood Safetypt_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectGenetic Diversitypt_PT
dc.subjectBiofilm Formationpt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectResistência aos Antimicrobianos
dc.titleListeria monocytogenes from Food Products and Food Associated Environments: Antimicrobial Resistance, Genetic Clustering and Biofilm Insightspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00772%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage447pt_PT
oaire.citation.titleJournal of Antibiotics
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione683cb7c-d293-4738-83c6-c3c95d798e4d
relation.isProjectOfPublication.latestForDiscoverye683cb7c-d293-4738-83c6-c3c95d798e4d

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