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Evaluation of cytotoxicity and genotoxicity induced by oleic acid-coated iron oxide nanoparticles in human astrocytes

2019-08-30Journal article Restricted access
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dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorCosta, Carla
dc.contributor.authorBrandão, Fátima
dc.contributor.authorDuarte, José Alberto
dc.contributor.authorTeixeira, Joao Paulo
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorLaffon, Blanca
dc.date.accessioned2020-05-08T16:11:58Z
dc.date.available2020-05-08T16:11:58Z
dc.date.issued2019-08-30
dc.description.abstractIron oxide nanoparticles (ION) are gaining importance as diagnostic and therapeutic tool of central nervous system diseases. Although oleic acid-coated ION (O-ION) have been described as stable and biocompatible, their potential neurotoxicity was scarcely evaluated in human nervous cells so far. The primary aim of this work was to assess the molecular and cellular effects of O-ION on human astrocytes (A172 cells) under different experimental conditions. An extensive set of cyto- and genotoxicity tests was carried out, including lactate dehydrogenase release assay, cell cycle alterations, and cell death production, as well as comet assay, γH2AX assay, and micronucleus (MN) test, considering also iron ion release capacity and alterations in DNA repair ability. Results showed a moderate cytotoxicity related to cell cycle arrest and cell death promotion, regardless of serum presence. O-ION induced genotoxic effects, namely primary DNA damage, as detected by the comet assay and H2AX phosphorylation, but A172 cells were able to repair this particular damage because no chromosome alterations were found (confirmed by MN test results). Accordingly, no effects on the DNA repair ability were observed. The presence of serum proteins did not influence O-ION toxicity. Iron ions released from the O-ION surface seemed not to be responsible for the cytotoxic and genotoxic effects observed. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.pt_PT
dc.description.sponsorshipConsellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia. Grant Number: ED481B 2016/190‐0 Fundação para a Ciência e a Tecnologia. Grant Number: SFRH/BD101060/2014 Deputación Provincial da Coruña. Grant Number: 2019000011550 Ministerio de Educación, Cultura y Deporte. Grant Number: PRX19/00353 Xunta de Galicia. Grant Number: ED431B 2019/02pt_PT
dc.description.sponsorshipThis work was funded by Xunta de Galicia (ED431B 2019/02). Vanessa Valdiglesias was supported by a Xunta de Galicia postdoctoral fellowship (ED481B 2016/190-0), B. Laffon by a Ministerio de Educación, Cultura y Deporte fellowship (PRX19/00353), Natalia Fernández-Bertólez by a Deputación Provincial da Coruña fellowship (2019000011550), and Fátima Brandao by the Portuguese Foundation for Science and Technology (SFRH/BD101060/2014). Authors would also like to acknowledge COST Action CA15132 “The comet assay as a human biomonitoring tool (hCOMET).”
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEnviron Mol Mutagen. 2019 Nov 13;60(9):816-829. doi: 10.1002/em.22323. Epub 2019 Aug 30pt_PT
dc.identifier.doi10.1002/em.22323pt_PT
dc.identifier.issn0893-6692
dc.identifier.urihttp://hdl.handle.net/10400.18/6619
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/em.22323pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAstrocytespt_PT
dc.subjectCell Cycle Checkpointspt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectCell Survivalpt_PT
dc.subjectChromosome Aberrationspt_PT
dc.subjectComet Assaypt_PT
dc.subjectDNA Damagept_PT
dc.subjectDNA Repairpt_PT
dc.subjectFerric Compoundspt_PT
dc.subjectHumanspt_PT
dc.subjectMetal Nanoparticlespt_PT
dc.subjectMicronucleus Testspt_PT
dc.subjectMutagenicity Testspt_PT
dc.subjectMutagenspt_PT
dc.subjectOleic Acidpt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleEvaluation of cytotoxicity and genotoxicity induced by oleic acid-coated iron oxide nanoparticles in human astrocytespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage829pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage816pt_PT
oaire.citation.titleEnvironmental and Molecular Mutagenesispt_PT
oaire.citation.volume60pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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