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Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)

2021-06-07Journal article Open access
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dc.contributor.authorBrandão, Fátima
dc.contributor.authorCosta, Carla
dc.contributor.authorBessa, Maria João
dc.contributor.authorDumortier, Elise
dc.contributor.authorDebacq-Chainiaux, Florence
dc.contributor.authorHubaux, Roland
dc.contributor.authorSalmon, Michel
dc.contributor.authorLaloy, Julie
dc.contributor.authorStan, Miruna S.
dc.contributor.authorHermenean, Anca
dc.contributor.authorGharbia, Sami
dc.contributor.authorDinischiotu, Anca
dc.contributor.authorBannuscher, Anne
dc.contributor.authorHellack, Bryan
dc.contributor.authorHaase, Andrea
dc.contributor.authorFraga, Sónia
dc.contributor.authorTeixeira, João
dc.date.accessioned2022-02-01T16:25:44Z
dc.date.available2022-02-01T16:25:44Z
dc.date.issued2021-06-07
dc.descriptionThis article belongs to the Special Issue Cytotoxicity and Genotoxicity of Nanomaterials.
dc.description.abstractSeveral reports on amorphous silica nanomaterial (aSiO2 NM) toxicity have been questioning their safety. Herein, we investigated the in vivo pulmonary toxicity of four variants of aSiO2 NM: SiO2_15_Unmod, SiO2_15_Amino, SiO2_7 and SiO2_40. We focused on alterations in lung DNA and protein integrity, and gene expression following single intratracheal instillation in rats. Additionally, a short-term inhalation study (STIS) was carried out for SiO2_7, using TiO2_NM105 as a benchmark NM. In the instillation study, a significant but slight increase in oxidative DNA damage in rats exposed to the highest instilled dose (0.36 mg/rat) of SiO2_15_Amino was observed in the recovery (R) group. Exposure to SiO2_7 or SiO2_40 markedly increased oxidative DNA lesions in rat lung cells of the exposure (E) group at every tested dose. This damage seems to be repaired, since no changes compared to controls were observed in the R groups. In STIS, a significant increase in DNA strand breaks of the lung cells exposed to 0.5 mg/m3 of SiO2_7 or 50 mg/m3 of TiO2_NM105 was observed in both groups. The detected gene expression changes suggest that oxidative stress and/or inflammation pathways are likely implicated in the induction of (oxidative) DNA damage. Overall, all tested aSiO2 NM were not associated with marked in vivo toxicity following instillation or STIS. The genotoxicity findings for SiO2_7 from instillation and STIS are concordant; however, changes in STIS animals were more permanent/difficult to revert.pt_PT
dc.description.sponsorshipThis work was funded by the Portuguese Foundation for Science and Technology (FCT) through ERA-NET SIINN project NanoToxClass (SIINN/0001/2013). FB and MJB are recipients of FCT PhD scholarships (SFRH/BD/101060/2014 and SFRH/BD/12046/2016). Thanks are due to FCT/MCTES for the financial support through national funds to EPIUnit (UIDB/04750/2020). J. Laloy performed STIS at the University of Namur with funding provided by BfR (grant agreement number 1329-561). F. Debacq-Chainiaux is a research associate at FRS-FNRS (National Funds for Scientific Research, Belgium).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanomaterials (Basel). 2021 Jun 7;11(6):1502. doi: 10.3390/nano11061502pt_PT
dc.identifier.doi10.3390/nano11061502pt_PT
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10400.18/7909
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationSFRH/BD/12046/2016pt_PT
dc.relationEstablishing nanomaterial grouping/ classification strategies according to toxicity and biological effects for supporting risk assessment
dc.relationEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
dc.relationEpidemiology Research Unit - Institute of Public Health, University of Porto
dc.relation.publisherversionhttps://www.mdpi.com/2079-4991/11/6/1502pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectToxicologypt_PT
dc.subjectSilica Nanomaterialspt_PT
dc.subjectIn vivo Inhalationpt_PT
dc.subjectIn vivo Instillationpt_PT
dc.subjectRat Lungpt_PT
dc.subjectDNA Damagept_PT
dc.subjectGene Expressionpt_PT
dc.subjectNanomaterialspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleGenotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEstablishing nanomaterial grouping/ classification strategies according to toxicity and biological effects for supporting risk assessment
oaire.awardTitleEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
oaire.awardTitleEpidemiology Research Unit - Institute of Public Health, University of Porto
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/ERA-SIINN%2F0001%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F101060%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1502pt_PT
oaire.citation.titleNanomaterials (Basel)pt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePinto Brandão
person.familyNameCosta
person.familyNameBessa
person.familyNameFraga
person.familyNamePaulo Teixeira
person.givenNameMaria de Fátima
person.givenNameCarla
person.givenNameMaria João
person.givenNameSónia
person.givenNameJoao
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person.identifier.orcid0000-0002-2933-8561
person.identifier.orcid0000-0002-0027-8462
person.identifier.orcid0000-0001-5357-6167
person.identifier.orcid0000-0001-9386-2336
person.identifier.orcid0000-0001-8693-5250
person.identifier.ridK-4048-2014
person.identifier.ridF-5445-2013
person.identifier.scopus-author-id55984029000
person.identifier.scopus-author-id7005283366
person.identifier.scopus-author-id36097134700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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