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Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages

2023-08-21Journal article Open access
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dc.contributor.authorVentura, Célia
dc.contributor.authorPinto, Fátima
dc.contributor.authorLourenço, Ana Filipa
dc.contributor.authorPedrosa, Jorge F. S.
dc.contributor.authorFernandes, Susete N.
dc.contributor.authorda Rosa, Rafaela R.
dc.contributor.authorGodinho, Maria Helena
dc.contributor.authorFerreira, Paulo J. T.
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2023-10-04T10:46:35Z
dc.date.available2023-10-04T10:46:35Z
dc.date.issued2023-08-21
dc.description.abstractCellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicity is an endpoint that must be assessed to ensure that no carcinogenic risks are associated with exposure to nanomaterials. In this study, we evaluated the genotoxicity of two types of cellulose micro/nanofibrils (CMF and CNF) and one sample of cellulose nanocrystals (CNC), obtained from industrial bleached Eucalyptus globulus kraft pulp. For that, we exposed co-cultures of human alveolar epithelial A549 cells and THP-1 monocyte-derived macrophages to a concentration range of each CMNM and used the micronucleus (MN) and comet assays. Our results showed that only the lowest concentrations of the CMF sample were able to induce DNA strand breaks (FPG-comet assay). However, none of the three CMNMs produced significant chromosomal alterations (MN assay). These findings, together with results from previous in vitro studies using monocultures of A549 cells, indicate that the tested CNF and CNC are not genotoxic under the conditions tested, while the CMF display a low genotoxic potential.pt_PT
dc.description.sponsorshipThe work was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The authors also acknowledge the support of FCT/MCTES to the following projects: LA/P/0037/2020, UIDP/50025/2020 and UIDB/50025/2020 of the Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication-i3N, CIEPQPF (UIDB/00102/2020) and ToxOmics, NMS-UNL (UIDP/00009/2020; UIDB/00009/2020). Thanks also to European Cooperation in Science & Technology (COST) Action: CA21159—Understanding interaction light—biological surfaces: possibility for new electronic materials and devices (PhoBioS); Inpactus Project—innovative products and technologies from eucalyptus (Project No. 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 No. 246/AXIS II/2017).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBioengineering (Basel). 2023 Aug 21;10(8):986. doi: 10.3390/bioengineering10080986pt_PT
dc.identifier.doi10.3390/bioengineering10080986pt_PT
dc.identifier.issn2306-5354
dc.identifier.urihttp://hdl.handle.net/10400.18/8651
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0037/2020pt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationChemical Process Engineering and Forest Products Research Centre
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.relation.publisherversionhttps://www.mdpi.com/2306-5354/10/8/986pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectNanofibrillated Cellulosept_PT
dc.subjectBiocompatibilitypt_PT
dc.subjectNanocrystalline Cellulosept_PT
dc.subjectSafety Assessmentpt_PT
dc.subjectRespiratory Effectspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleAssessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleChemical Process Engineering and Forest Products Research Centre
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
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oaire.citation.issue8pt_PT
oaire.citation.startPage986pt_PT
oaire.citation.titleBioengineeringpt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVentura
person.familyNameLouro
person.familyNameSilva
person.givenNameCélia
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier2056460
person.identifier157627
person.identifier.ciencia-id6116-89BA-C617
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0002-0637-2222
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com a política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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