Repository logo
 
Publication

The role of Rhipicephalus sanguineus sensu lato saliva in the dissemination of Rickettsia conorii in C3H/HeJ mice

2015-09-29Journal article Restricted access
Simple item page
dc.contributor.authorMilhano, N.
dc.contributor.authorSaito, T.B.
dc.contributor.authorBechelli, J.
dc.contributor.authorFang, R.
dc.contributor.authorVilhena, M.
dc.contributor.authorDe Sousa, Rita
dc.contributor.authorWalker, D.H.
dc.date.accessioned2016-02-18T14:30:16Z
dc.date.available2016-09-01T00:30:08Z
dc.date.issued2015-09-29
dc.description.abstractAnimal models have been developed for the study of rickettsial pathogenesis. However, to understand what occurs during the natural route of rickettsial transmission via the tick bite, the role of tick saliva should be considered in these models. To address this, we analysed the role of tick saliva in the transmission of Rickettsia conorii (Rickettsiales: Rickettsiaceae) in a murine host by intradermally (i.d.) inoculating two groups of susceptible C3H/HeJ mice with this Rickettsia, and infesting one group with nymphal Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae) ticks. Quantification of bacterial loads and mRNA levels of interleukin-1β (IL-1β), IL-10 and NF-κB was performed in C3H/HeJ lung samples by real-time quantitative polymerase chain reaction (PCR) and real-time reverse transcriptase PCR, respectively. Lung histology was examined to evaluate the pathological manifestations of infection. No statistically significant difference in bacterial load in the lungs of mice was observed between these two groups; however, a statistically significant difference was observed in levels of IL-1β and NF-κB, both of which were higher in the group inoculated with rickettsiae but not infected with ticks. Lung histology in both groups of animals revealed infiltration of inflammatory cells. Overall, this study showed that i.d. inoculation of R. conorii caused infection in the lungs of C3H/HeJ mice and tick saliva inhibited proinflammatory effects.pt_PT
dc.identifier.citationMed Vet Entomol. 2015 Sep;29(3):225-9. doi: 10.1111/mve.12118. Epub 2015 May 26pt_PT
dc.identifier.doi10.1111/mve.12118pt_PT
dc.identifier.issn0269-283X
dc.identifier.urihttp://hdl.handle.net/10400.18/3407
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley/ Royal Entomological Societypt_PT
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/mve.12118/fullpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.subjectRhipicephalus Sanguineus sensu latopt_PT
dc.subjectRickettsia Conoriipt_PT
dc.subjectIntradermal Inoculationpt_PT
dc.subjectReal-time PCRpt_PT
dc.subjectTick Salivapt_PT
dc.titleThe role of Rhipicephalus sanguineus sensu lato saliva in the dissemination of Rickettsia conorii in C3H/HeJ micept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage229pt_PT
oaire.citation.startPage225pt_PT
oaire.citation.titleMedical and Veterinary Entomologypt_PT
oaire.citation.volume29(3)pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
N. Milhano The role of Rhipicephalus sanguineus....pdf
Size:
183.12 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais