Repository logo
 
Publication

Tolerance to multiple metal stressors in emerging non-typhoidal MDR Salmonella serotypes: a relevant role for copper in anaerobic conditions

2016-08Journal article Restricted access
Simple item page
dc.contributor.authorMourão, Joana
dc.contributor.authorMarçal, Sara
dc.contributor.authorRamos, Paula
dc.contributor.authorCampos, Joana
dc.contributor.authorMachado, Jorge
dc.contributor.authorPeixe, Luísa
dc.contributor.authorNovais, Carla
dc.contributor.authorAntunes, Patrícia
dc.date.accessioned2017-03-03T13:02:54Z
dc.date.available2017-03-03T13:02:54Z
dc.date.issued2016-08
dc.description.abstractObjectives: Factors driving the expansion of particular MDR Salmonella serotypes/clones are not completely understood. We assessed if emergent MDR Salmonella serotypes/clones were more enriched in metal tolerance genes (e.g. to Cu/Ag) than other less frequent ones, as an additional feature to survive in environments contaminated with metals. Methods: Metal (Cu pco/Ag,Cu sil/Hg mer/As ars/Te ter) tolerance genes screening (PCR/sequencing), MICs of CuSO4/AgNO3 (aerobiosis/anaerobiosis), genetic element characterization (S1/I-CeuI PFGE) and conjugation assays were performed in a well-characterized Salmonella collection (n = 275 isolates; 2000–14; 49 serotypes/clones). Results The sil ± pco genes were detected in 37% of isolates from diverse serotypes, mainly in emergent Rissen/ST469 and Typhimurium/ST34 European clone (100%), which are mostly associated with pig settings where Cu is highly used. These genes were frequently co-located with merA ± terF and/or antibiotic resistance genes in plasmids (100–270 kb; IncHI2/IncHI1/IncN/IncFIIA; mostly transferable by conjugation) or in the chromosome. Most sil ± pco+ isolates (77%) were MDR contrasting with sil ± pco− ones (48%). The sil ± pco+ isolates presented significantly higher MICCuSO4 under anaerobiosis (MIC50/MIC90 = 28/32 mM) and MICAgNO3 after previous Ag contact (MIC50/MIC90 > 3 mM) than sil− ones (MIC50/MIC90 = 2/8 mM to CuSO4; MIC50/MIC90 = 0.125/0.16 mM to AgNO3). Use of these modified methodological approaches allowed the establishment of CuSO4/AgNO3 tolerance cut-offs to differentiate sil+ and sil− isolates, here firstly proposed. Conclusions: This study demonstrates that acquisition of Cu/Ag tolerance genes (sil/pco genes) might contribute to the emergence of particular clinically relevant MDR Salmonella serotypes/clones by facilitating their survival in diverse metal-contaminated settings, particularly in pig production. Assessment of control measures for the use and/or accumulation of metals in diverse environments are needed to prevent a wider expansion of such strains or the emergence of new ones.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Cência e a Tecnologia (FCT)/Ministério da Educação e Ciência (MEC) through national funds and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER), under the Partnership Agreement PT2020, with the reference UID/MULTI/04378/2013 – POCI/01/0145/FERDER/007728. J. Moura˜o and J. C. (grant numbers SFRH/BD/77518/2011 and SFRH/BD/93091/2013, respectively; POPH-QREN) were supported by PhD fellowships from FCT. We are greatly indebted to all of the financing sources.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Antimicrob Chemother. 2016 Aug;71(8):2147-57. doi: 10.1093/jac/dkw120. Epub 2016 Apr 26.pt_PT
dc.identifier.doi10.1093/jac/dkw120pt_PT
dc.identifier.issn0305-7453
dc.identifier.urihttp://hdl.handle.net/10400.18/4431
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Press/British Society for Antimicrobial Chemotherapypt_PT
dc.relation.publisherversionhttps://academic.oup.com/jac/article-abstract/71/8/2147/2238170/Tolerance-to-multiple-metal-stressors-in-emerging?redirectedFrom=fulltextpt_PT
dc.subjectSalmonellapt_PT
dc.subjectMDRpt_PT
dc.subjectSerotypespt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleTolerance to multiple metal stressors in emerging non-typhoidal MDR Salmonella serotypes: a relevant role for copper in anaerobic conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.citation.endPage2157pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage2147pt_PT
oaire.citation.titleJournal of Antimicrobial Chemotherapypt_PT
oaire.citation.volume71pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication72797dcd-bef0-4686-8fd4-4ffc88f8cb5b
relation.isProjectOfPublication.latestForDiscovery72797dcd-bef0-4686-8fd4-4ffc88f8cb5b

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Morão et al_2016.pdf
Size:
295.45 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais