Repository logo
 
Publication

Exposure assessment in one central hospital: A multi-approach protocol to achieve an accurate risk characterization

2019-11-18Journal article Restricted access
Simple item page
dc.contributor.authorViegas, Carla
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorMonteiro, Ana
dc.contributor.authorPaciência, Inês
dc.contributor.authorRufo, João
dc.contributor.authorAguiar, Lívia
dc.contributor.authorLage, Bruna
dc.contributor.authorDiogo Gonçalves, Lídia Maria
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Quintal
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorKosicki, Robert
dc.contributor.authorGrajewski, Jan
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorViegas, Susana
dc.contributor.authorPereira, Cristiana
dc.date.accessioned2020-05-11T07:36:44Z
dc.date.available2020-05-11T07:36:44Z
dc.date.issued2019-11-18
dc.description.abstractThe bioburden in a Hospital building originates not only from patients, visitors and staff, but is also disseminated by several indoor hospital characteristics and outdoor environmental sources. This study intends to assess the exposure to bioburden in one central Hospital with a multi-approach protocol using active and passive sampling methods. The microbial contamination was also characterized through molecular tools for toxigenic species, antifungal resistance and mycotoxins and endotoxins profile. Two cytotoxicity assays (MTT and resazurin) were conducted with two cell lines (Calu-3 and THP-1), and in vitro pro-inflammatory potential was assessed in THP-1 cell line. Out of the 15 sampling locations 33.3% did not comply with Portuguese legislation regarding bacterial contamination, whereas concerning fungal contamination 60% presented I/O > 1. Toxigenic fungal species were observed in 27% of the sampled rooms (4 out of 15) and qPCR analysis successfully amplified DNA from the Aspergillus sections Flavi and Fumigati, although mycotoxins were not detected. Growth of distinct fungal species was observed on Sabouraud dextrose agar with triazole drugs, such as Aspergillus section Versicolores on 1 mg/L VORI. The highest concentrations of endotoxins were found in settled dust samples and ranged from 5.72 to 23.0 EU.mg-1. While a considerable cytotoxic effect (cell viability < 30%) was observed in one HVAC filter sample with Calu-3 cell line, it was not observed with THP-1 cell line. In air samples a medium cytotoxic effect (61-68% cell viability) was observed in 3 out of 15 samples. The cytokine responses produced a more potent average cell response (46.8 ± 12.3 ρg/mL IL-1β; 90.8 ± 58.5 ρg/mL TNF-α) on passive samples than air samples (25.5 ± 5.2 ρg/mL IL-1β and of 19.4 ± 5.2 ρg/mL TNF-α). A multi-approach regarding parameters to assess, sampling and analysis methods should be followed to characterize the biorburden in the Hospital indoor environment. This study supports the importance of considering exposure to complex mixtures in indoor environments.pt_PT
dc.description.abstractHighlights: The multi-parameter approach on sampling methods and assays allowed to assess the biorburden; Toxigenic fungal species were identified by culture based-methods and detected by qPCR; Aspergillus section Versicolores was identified in the resistance to azoles screening; Low levels of endotoxins were observed in the environmental matrices; Low to moderate cytotoxic and pro-inflammatory effects were found.pt_PT
dc.description.sponsorshipThis work was supported by FCT – Fundação para Ciência e Tecnologia for funding the project EXPOsE – Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/ SAICT/2016 – Project nº 23222). The project is co-financed by FCT – Fundação para Ciência e Tecnologia, Portugal and by the Polish Minister of Science and Higher Education, under 664 the program "Regional Initiative of Excellence" in 2019 - 2022 (Grant No.665 008/ RID/2018/19).
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEnviron Res. 2020 Feb;181:108947. doi: 10.1016/j.envres.2019.108947. Epub 2019 Nov 18pt_PT
dc.identifier.doi10.1016/j.envres.2019.108947pt_PT
dc.identifier.issn0013-9351
dc.identifier.urihttp://hdl.handle.net/10400.18/6645
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Academic Presspt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0013935119307443?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectBioburdenpt_PT
dc.subjectAzole Resistancept_PT
dc.subjectEndotoxinspt_PT
dc.subjectHospitalpt_PT
dc.subjectActive and Passive Sampling Methodspt_PT
dc.subjectCytotoxicity Assessmentpt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleExposure assessment in one central hospital: A multi-approach protocol to achieve an accurate risk characterizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage108947pt_PT
oaire.citation.titleEnvironmental Researchpt_PT
oaire.citation.volume181pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Exposure.pdf
Size:
766.98 KB
Format:
Adobe Portable Document Format
Download

Collections

DSA - Artigos em revistas internacionais