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Simultaneous Extraction and Determination of Preservatives and Antioxidants in Juice Samples by an Optimized Microextraction Method Using Central Composite Design and Validated with Accuracy Profile

2018-06-28Journal article Restricted access
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dc.contributor.authorTighrine, Abderrahmane
dc.contributor.authorPinto, Edgar
dc.contributor.authorMelo, Armindo
dc.contributor.authorFerreira, Isabel M.P.L.V.O.
dc.contributor.authorMamou, Marzouk
dc.contributor.authorAmir, Youcef
dc.date.accessioned2019-03-27T11:45:13Z
dc.date.available2019-03-27T11:45:13Z
dc.date.issued2018-06-28
dc.description.abstractBackground: Food additives are widely used in industries. Overall, these additives have a beneficial role, but if their concentration exceeds certain limits, they may have an adverse effect on human health. Objective: This study outlines the determination of benzoic acid (BA), sorbic acid (SA), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) in juices using dispersive liquid-liquid microextraction and HPLC-diode-array detection. Methods: Different parameters that significantly affect the extraction efficiency were optimized. The disperser and extraction solvents were acetone and chloroform, respectively. The other parameters were selected and optimized using two-level (2k) factorial and central composite designs, respectively. A full method validation using an accuracy profile for the total measurement error was carried out. Results: The optimized conditions were 625 μL acetone, 350 μL chloroform, 0.85 g NaCl, and pH 2. The validated method demonstrated good linearity with R² ≥ 0.99 and good precision with the RSD of repeatability and intermediate precision at 1.97-10.7% and 2.31-11.9%, respectively. The relative recoveries were 96.2-105.4%, and the LODs were around 0.03 μg/mL for all compounds. The analysis of 41 samples revealed that BA and SA were quantified in 25 and 20 samples, respectively. BHA and BHT were not detected. Conclusions: The proposed methodology is an appropriate tool to determine important food additives in juices to ensure their compliance with the food additives legislation. Highlights: The work has demonstrated that the method can be successfully used in the simultaneous determination of four food additives in fruit juices.pt_PT
dc.description.sponsorshipThis work received financial support from the European Union (FEDER funds POCI/01/0145/FEDER/007265) and National Funds (FCT/MEC, Fundação para a Ciência e Tecnologia and Ministério da Educação e Ciência) under the Partnership Agreement PT2020 UID/QUI/50006/2013.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ AOAC Int. 2018 Jun 28. doi: 10.5740/jaoacint.18-0104. [Epub ahead of print]pt_PT
dc.identifier.doi10.5740/jaoacint.18-0104pt_PT
dc.identifier.issn1060-3271
dc.identifier.urihttp://hdl.handle.net/10400.18/6326
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAOAC Internationalpt_PT
dc.relation.publisherversionhttps://www.ingentaconnect.com/content/aoac/jaoac/2019/00000102/00000001/art00024;jsessionid=3vkwb5pl1cqq3.x-ic-live-01pt_PT
dc.subjectFood Additivespt_PT
dc.subjectFruit Juicespt_PT
dc.subjectBenzoic Acidpt_PT
dc.subjectSorbic Acidpt_PT
dc.subjectButylated Hydroxyanisolept_PT
dc.subjectButylated Hydroxytoluenept_PT
dc.subjectComposição dos Alimentospt_PT
dc.titleSimultaneous Extraction and Determination of Preservatives and Antioxidants in Juice Samples by an Optimized Microextraction Method Using Central Composite Design and Validated with Accuracy Profilept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage216pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage208pt_PT
oaire.citation.titleJournal of AOAC Internationalpt_PT
oaire.citation.volume102pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationd7883dc7-6335-4f26-8bd0-e2ea4bb07c07
relation.isProjectOfPublication.latestForDiscoveryd7883dc7-6335-4f26-8bd0-e2ea4bb07c07

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