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Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy

2016-03-08Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorFaria, Tiago
dc.contributor.authordos Santos, Mateus
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorSabino, Raquel
dc.contributor.authorQuintal Gomes, Anita
dc.contributor.authorViegas, Susana
dc.date.accessioned2017-02-17T16:05:24Z
dc.date.available2017-02-17T16:05:24Z
dc.date.issued2016-03-08
dc.descriptionFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808960/pt_PT
dc.description.abstractIn slaughterhouses, the biological risk is present not only from the direct or indirect contact with animal matter, but also from the exposure to bioaerosols. Fungal contamination was already reported from the floors and walls of slaughterhouses. This study intends to assess fungal contamination by cultural and molecular methods in poultry, swine/bovine and large animal slaughterhouses. Air samples were collected through an impaction method, while surface samples were collected by the swabbing method and subjected to further macro- and micro-scopic observations. In addition, we collected air samples using the impinger method in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely A. flavus, A. fumigatus and A. ochraceus complexes. Poultry and swine/bovine slaughterhouses presented each two sampling sites that surpass the guideline of 150 CFU/m3. Scopulariopsis candida was the most frequently isolated (59.5%) in poultry slaughterhouse air; Cladosporium sp. (45.7%) in the swine/bovine slaughterhouse; and Penicillium sp. (80.8%) in the large animal slaughterhouse. Molecular tools successfully amplified DNA from the A. fumigatus complex in six sampling sites where the presence of this fungal species was not identified by conventional methods. This study besides suggesting the indicators that are representative of harmful fungal contamination, also indicates a strategy as a protocol to ensure a proper characterization of fungal occupational exposure.pt_PT
dc.description.sponsorshipThis study was supported by the Environment & Health Research Group from Lisbon School of Health Technology and Associação Para o Desenvolvimento de Conhecimento e Inovação (POLITEC & ID).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Environ Res Public Health. 2016 Mar 8;13(3). pii: E297. doi: 10.3390/ijerph13030297pt_PT
dc.identifier.doi10.3390/ijerph13030297pt_PT
dc.identifier.issn1660-4601
dc.identifier.urihttp://hdl.handle.net/10400.18/4277
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttp://www.mdpi.com/1660-4601/13/3/297pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFungal Burdenpt_PT
dc.subjectAssessment Strategypt_PT
dc.subjectSlaughterhousespt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleSlaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPageE297pt_PT
oaire.citation.titleInternational Journal of Environmental Research and Public Healthpt_PT
oaire.citation.volume13(3)pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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