Repository logo
 
Publication

In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures

2021-11-27Journal article Open access
Simple item page
dc.contributor.authorBessa, Maria João
dc.contributor.authorBrandão, Fátima
dc.contributor.authorFokkens, Paul H.B.
dc.contributor.authorLeseman, Daan L.A.C.
dc.contributor.authorBoere, A. John F.
dc.contributor.authorCassee, Flemming R.
dc.contributor.authorSalmatonidis, Apostolos
dc.contributor.authorViana, Mar
dc.contributor.authorVulpoi, Adriana
dc.contributor.authorSimon, Simion
dc.contributor.authorMonfort, Eliseo
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorFraga, Sónia
dc.date.accessioned2022-02-02T15:48:46Z
dc.date.available2022-02-02T15:48:46Z
dc.date.issued2021-11-27
dc.descriptionThis article belongs to the Special Issue Engineered Nanomaterials Exposure and Risk Assessment: Occupational Health and Safetypt_PT
dc.description.abstractDiverse industries have already incorporated within their production processes engineered nanoparticles (ENP), increasing the potential risk of worker inhalation exposure. In vitro models have been widely used to investigate ENP toxicity. Air-liquid interface (ALI) cell cultures have been emerging as a valuable alternative to submerged cultures as they are more representative of the inhalation exposure to airborne nano-sized particles. We compared the in vitro toxicity of four ENP used as raw materials in the advanced ceramics sector in human alveolar epithelial-like cells cultured under submerged or ALI conditions. Submerged cultures were exposed to ENP liquid suspensions or to aerosolised ENP at ALI. Toxicity was assessed by determining LDH release, WST-1 metabolisation and DNA damage. Overall, cells were more sensitive to ENP cytotoxic effects when cultured and exposed under ALI. No significant cytotoxicity was observed after 24 h exposure to ENP liquid suspensions, although aerosolised ENP clearly affected cell viability and LDH release. In general, all ENP increased primary DNA damage regardless of the exposure mode, where an increase in DNA strand-breaks was only detected under submerged conditions. Our data show that at relevant occupational concentrations, the selected ENP exert mild toxicity to alveolar epithelial cells and exposure at ALI might be the most suitable choice when assessing ENP toxicity in respiratory models under realistic exposure conditions.pt_PT
dc.description.sponsorshipThis research was funded by CERASAFE (www.cerasafe.eu; accessed on 26 October 2021), with the support of ERA-NET SIINN (project id:16) and the Portuguese Foundation for Science and Technology (FCT; SIINN/0004/2014). This work was also supported by the NanoBioBarriers project (PTDC/MED-TOX/31162/2017), co-financed by the Operational Program for Competitiveness and Internationalization (POCI) through European Regional Development Funds (FEDER/FNR) and FCT; Spanish Ministry of Science and Innovation (projects PCIN-2015-173-C02-01 and CEX2018-000794- S-Severo Ochoa), and by the Romanian National Authority for Scientific Research and Innovation (CCCDI-UEFISCDI, project number 29/2016 within PNCDI III). M.J. Bessa (SFRH/BD/120646/2016) and F. Brandão (SFRH/BD/101060/2014) are recipients of FCT PhD scholarships under the framework of Human Capital Operating Program (POCH) and European Union funding. The Doctoral Program in Biomedical Sciences, of the ICBAS—University of Porto, offered additional funds. S. Fraga thanks FCT for funding through program DL 57/2016–Norma transitória (Ref. DL-57/INSA-06/2018). Thanks are also due to FCT/MCTES for the financial support to EPIUnit (UIDB/04750/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanomaterials (Basel). 2021 Nov 27;11(12):3225. doi: 10.3390/nano11123225.pt_PT
dc.identifier.doi10.3390/nano11123225pt_PT
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10400.18/7912
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationSAFE PRODUCTION AND USE OF NANOMATERIALS IN THE CERAMIC INDUSTRY
dc.relationAssessing the toxicity of airborne nanoceramics in vitro and in vivo- contribution to the safe production and use of nanomaterials in the ceramic industry
dc.relationEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
dc.relationEpidemiology Research Unit - Institute of Public Health, University of Porto
dc.relation.publisherversionhttps://www.mdpi.com/2079-4991/11/12/3225pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDNA Damagept_PT
dc.subjectAir-Liquid Interfacept_PT
dc.subjectEngineered Nanoparticlespt_PT
dc.subjectNanoparticlespt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectIn Vitro Cytotoxicitypt_PT
dc.subjectSubmerged Culturespt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleIn Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Culturespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSAFE PRODUCTION AND USE OF NANOMATERIALS IN THE CERAMIC INDUSTRY
oaire.awardTitleAssessing the toxicity of airborne nanoceramics in vitro and in vivo- contribution to the safe production and use of nanomaterials in the ceramic industry
oaire.awardTitleEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
oaire.awardTitleEpidemiology Research Unit - Institute of Public Health, University of Porto
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/SIINN%2F0004%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F120646%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F101060%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT
oaire.citation.issue12pt_PT
oaire.citation.startPage3225pt_PT
oaire.citation.titleNanomaterialspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication11b35b04-d00f-44df-99c1-21b221144573
relation.isProjectOfPublication1370de00-d228-47a7-8aed-320831dc4dcd
relation.isProjectOfPublication28045083-dc0a-40cf-9538-c1ef7ff6784e
relation.isProjectOfPublication8bcb6d0f-fda0-470a-906d-c67c257beedb
relation.isProjectOfPublication05f4f337-eeba-4c07-82ed-4202b21618a2
relation.isProjectOfPublication.latestForDiscovery11b35b04-d00f-44df-99c1-21b221144573

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nanomaterials-11-03225-v2.pdf
Size:
3.16 MB
Format:
Adobe Portable Document Format
Download

Collections

DSA - Artigos em revistas internacionais