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Exploring potential genotoxic effects of nanocelluloses versus multi-walled carbon nanotubes in co-cultures of human lung epithelial cells and monocyte-derived macrophages

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dc.contributor.authorPinto, Fátima
dc.contributor.authorVentura, Célia
dc.contributor.authorTeixeira, Sara
dc.contributor.authorLourenço, Ana Filipa
dc.contributor.authorFernandes, S.N.
dc.contributor.authorda Rosa, R.R.
dc.contributor.authorGodinho, M.H.
dc.contributor.authorFerreira, Paulo J.T.
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2022-01-10T16:44:21Z
dc.date.available2022-01-10T16:44:21Z
dc.date.issued2021-09-22
dc.description.abstractCellulose nanomaterials (CNMs) are advanced materials exhibiting unique properties for innumerous industrial and biomedical applications. Human exposure to CNMs has been equally growing, which raises some concern, given the similarity of some CNMs size and morphology with that of multiwalled carbon nanotubes (MWCNTs) that induce lung toxicity. The genotoxic effects of three CNMs produced from Eucalyptus globulus bleached kraft pulp through different methods and, hence, harbouring different physicochemical properties (two micro/nano- fibrillated and one nanocrystalline), were investigated in human alveolar (A549) cells co-cultured with monocyte- derived (THP-1) macrophages and in conventional A549 cultures. Two MWCNT differing in diameter, length and flexibility, NM 401 and NM 402 (JRC Repository), were used as references. None of the CNMs was toxic to A549 cells. The results of the in vitro micronucleus assay showed that exposure of A549 cells (1.5 to 50 μg/cm2 48h) to each CNM or to NM 402), either in monoculture or in co-culture, did not produce significant alterations in the frequency of micronucleated binucleated cells (MNBNC), as compared to the control. In contrast, NM-401, the thickest, longest and more rigid nanofiber, was able to significantly increase the frequencies of MNBNC. The cytokinesis-block proliferation index of A549 cells was not affected by CNMs or MWNTs exposure. To conclude, although data from other endpoints is needed, the present in vitro data suggests that the studied CNMs are neither toxic nor genotoxic to lung cells, increasing the weight of evidence in favor of their biocompatibility.pt_PT
dc.description.sponsorshipThe work was funded by FCT/MCTES through national funds (PIDDAC), project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017), UIDB/00102/2020 and UIDB/00009/2020; UIDP/00009/2020; FEDER, EU funds, through the COMPETE 2020 POCI and PORL, FCT/MCTES and POR Lisboa2020, under PIDDAC (POCI-01-0145-FEDER-007688) to projects UIDB/50025/2020-2023, PTDC/CTM-REF/30529/2017 and EUTOPIA CA17139.The authors acknowledge CYTED network NANOCELIA (Transferencia Tecnológica sobre aplicaciones de nanocelulosa en iberoamerica).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7845
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPTDC/SAU/PUB/32587/2017pt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectNanomaterialspt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectNanocellulosespt_PT
dc.subjectCellulose Nanomaterialspt_PT
dc.subjectHuman Lung Cellspt_PT
dc.subjectToxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleExploring potential genotoxic effects of nanocelluloses versus multi-walled carbon nanotubes in co-cultures of human lung epithelial cells and monocyte-derived macrophagespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTM-REF%2F30529%2F2017/PT
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.title52nd Annual Meeting Environmental Mutagenesis & Genomics Society (EMGS), 22-25 September 2021pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
person.familyNameLouro
person.familyNameSilva
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublicationa7763685-7c34-468d-b958-9dd0aca66db4
relation.isAuthorOfPublication.latestForDiscovery2361a951-8b9a-4b90-92d6-f6384003a242
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