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The harmonized INFOGEST in vitro digestion method: From knowledge to action

2015-12-08Journal article Restricted access
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dc.contributor.authorEgger, Lotti
dc.contributor.authorMenard, Olivia
dc.contributor.authorDelgado-Andrade, Cristina
dc.contributor.authorAlvito, Paula
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorBalance, Simon
dc.contributor.authorBarberá, Reyes
dc.contributor.authorBrodkorb, Andre
dc.contributor.authorCattenozh, Thomas
dc.contributor.authorClemente, Alfonso
dc.contributor.authorComi, Irene
dc.contributor.authorDupont, Didier
dc.contributor.authorGarcia-Llatas, Guadalupe
dc.contributor.authorLagarda, María Jesús
dc.contributor.authorLe Feunteun, Steven
dc.contributor.authorJanssenDuijghuijsen, Lonneke
dc.contributor.authorKarakaya, Sibel
dc.contributor.authorLesmes, Uri
dc.contributor.authorMackie, Alan R.
dc.contributor.authorMartins, Carla
dc.contributor.authorMeynier, Anne
dc.contributor.authorMiralles, Beatriz
dc.contributor.authorMurray, Brent S.
dc.contributor.authorPihlanto, Anne
dc.contributor.authorPicariello, Gianluca
dc.contributor.authorSantos, Claudia N.
dc.contributor.authorSimsekm, Sebnem
dc.contributor.authorRecio, Isidra
dc.contributor.authorRigby, Neil
dc.contributor.authorRioux, Laurie-Eve
dc.contributor.authorStoffers, Helena
dc.contributor.authorTavares, Ana
dc.contributor.authorTavares, Lucelia
dc.contributor.authorTurgeon, Sylvie
dc.contributor.authorUlleberg, Ellen K.
dc.contributor.authorVegarud, Gerd E.
dc.contributor.authorVergères, Guy
dc.contributor.authorPortmann, Reto
dc.date.accessioned2016-03-22T15:39:35Z
dc.date.available2018-01-01T01:30:10Z
dc.date.issued2015-12-08
dc.description.abstractWithin the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skimmilk powder (SMP) as amodel food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas β- lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The presentwork gives an overviewof all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future.pt_PT
dc.description.sponsorshipCOST Action FA1005 INFOGEST (http://www.cost-INFOGEST.eu/) is acknowledged for providing funding for travel, meetings, and conferences. The work was also supported by the MycoMix project (PTDC/ DTP-FTO/0417/2012), funded by the Fundação para a Ciência e Tecnologia (FCT, Portugal) and by iNOVA4Health: UID/Multi/04462/ 2013 and IF/01097/2013 from FCT.pt_PT
dc.identifier.citationFood Res Int. 2015. [Epub 2015 Dec 8]. doi:10.1016/j.foodres.2015.12.006pt_PT
dc.identifier.doi10.1016/j.foodres.2015.12.006pt_PT
dc.identifier.issn1873-7145
dc.identifier.urihttp://hdl.handle.net/10400.18/3704
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/Canadian Institute of Food Science and Technology (CIFST)pt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0963996915302751pt_PT
dc.subjectIn vitro Digestionpt_PT
dc.subjectDairy Proteinspt_PT
dc.subjectPeptidespt_PT
dc.subjectMass Spectrometrypt_PT
dc.subjectInter-laboratory Trialpt_PT
dc.subjectHarmonized IVD Protocolpt_PT
dc.subjectComposição dos Alimentospt_PT
dc.titleThe harmonized INFOGEST in vitro digestion method: From knowledge to actionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F0417%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04462%2F2013/PT
oaire.citation.titleFood Research Internationalpt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication7dee8a4a-7112-45ce-a8cb-8c552d7ae061
relation.isProjectOfPublicationf7e7f835-30c4-427e-a9ee-d5940c3e2710
relation.isProjectOfPublication.latestForDiscovery7dee8a4a-7112-45ce-a8cb-8c552d7ae061

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