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Challenges of the Application of In Vitro Digestion for Nanomaterials Safety Assessment

2024-05-28Research article Open access
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datacite.subject.fosCiências Naturais
dc.contributor.authorVital, Nádia
dc.contributor.authorGramacho, Ana Catarina
dc.contributor.authorSilva, Mafalda
dc.contributor.authorCardoso, Maria
dc.contributor.authorAlvito, Paula
dc.contributor.authorKranendonk, Michel
dc.contributor.authorSilva, Maria Joao
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2025-03-13T11:20:06Z
dc.date.available2025-03-13T11:20:06Z
dc.date.issued2024-05-28
dc.descriptionThis article belongs to the Special Issue Prospects for Risks and Benefits in the Context of Food and Health.
dc.description.abstractConsidering the increase in the production and use of nanomaterials (NM) in food/feed and food contact materials, novel strategies for efficient and sustainable hazard characterization, especially in the early stages of NM development, have been proposed. Some of these strategies encompass the utilization of in vitro simulated digestion prior to cytotoxic and genotoxic assessment. This entails exposing NM to fluids that replicate the three successive phases of digestion: oral, gastric, and intestinal. Subsequently, the resulting digestion products are added to models of intestinal cells to conduct toxicological assays, analyzing multiple endpoints. Nonetheless, exposure of intestinal cells to the digested products may induce cytotoxicity effects, thereby posing a challenge to this strategy. The aim of this work was to describe the challenges encountered with the in vitro digestion INFOGEST 2.0 protocol when using the digestion product in toxicological studies of NM, and the adjustments implemented to enable its use in subsequent in vitro biological assays with intestinal cell models. The adaptation of the digestion fluids, in particular the reduction of the final bile concentration, resulted in a reduced toxic impact of digestion products.
dc.description.sponsorshipThis research was funded by the Portuguese Fundação para a Ciência e a Tecnologia, I.P., through national funds PTDC/SAU-PUB/32587/2017 and PTDC/SAU-PUB/29481/2017. Research was co-funded by UIDB/00009/2020 (https://doi.org/10.54499/UIDP/00009/2020) and UIDP/00009/2020 (https://doi.org/10.54499/UIDB/00009/2020), both for the Centre for Toxicogenomics and Human Health—ToxOmics, FCT— Fundação para a Ciência e a Tecnologia. N.V. work was funded by Fundação para a Ciência e a Tecnologia, I.P PhD grant number 2020.07168.BD (https://doi.org/10.54499/2020.07168.BD). Thanks are due to CESAM by FCT/MCTES (UIDP/50017/2020+ UIDB/50017/2020 + LA/P/0094/2020), through national funds.
dc.identifier.citationVital N, Gramacho AC, Silva M, Cardoso M, Alvito P, Kranendonk M, Silva MJ, Louro H. Challenges of the Application of In Vitro Digestion for Nanomaterials Safety Assessment. Foods. 2024 May 28;13(11):1690. doi: 10.3390/foods13111690.
dc.identifier.doi10.3390/foods13111690
dc.identifier.issn2304-8158
dc.identifier.pmid38890918
dc.identifier.urihttp://hdl.handle.net/10400.18/10438
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
dc.relationCentre for Toxicogenomics and Human Health
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationUIDB/50017/2020
dc.relationLA/P/0094/2020
dc.relation.hasversionhttps://www.mdpi.com/2304-8158/13/11/1690
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectNanomaterials
dc.subjectCaco-2
dc.subjectDigestion Product
dc.subjectIn vitro Simulated Digestion
dc.subjectINFOGEST
dc.subjectFood Safety
dc.subjectEnvironmental Genotoxicity
dc.subjectGenotoxicidade Ambiental
dc.subjectSegurança Alimentar
dc.titleChallenges of the Application of In Vitro Digestion for Nanomaterials Safety Assessmentpor
dc.typeresearch article
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
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oaire.citation.issue11
oaire.citation.startPage1690
oaire.citation.titleFoods
oaire.citation.volume13
oaire.fundingStream3599-PPCDT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
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