Publication
How far ‘AUG-proximity effect’ goes?
| dc.contributor.author | Pereira, Francisco J.C. | |
| dc.contributor.author | Teixeira, Alexandre | |
| dc.contributor.author | Kong, Jian | |
| dc.contributor.author | Silva, Ana Luísa | |
| dc.contributor.author | Liebhaber, Stephen A. | |
| dc.contributor.author | Romão, Luísa | |
| dc.date.accessioned | 2012-10-24T15:51:30Z | |
| dc.date.available | 2012-10-24T15:51:30Z | |
| dc.date.issued | 2012-09-01 | |
| dc.description.abstract | Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature termination codons (PTCs). We have previously shown that mRNAs carrying a PTC located in close proximity to the translation initiation AUG codon escape NMD. This was called the “AUG-proximity effect”. The present work illustrates that the extension of the AUG-proximity effect, i.e. to what position in the open reading frame (ORF) an AUG-proximal PTC does not trigger NMD, is different between human a- and b-globin mRNAs. Remarkably, our data also demonstrate that, contrary to what occurs in the b-globin transcripts, a-globin mRNAs carrying an AUG-proximal PTC allow for efficient translation re-initiation, although it only partially explains their NMD resistance. In addition, our results reveal that in the a- and b-globin transcripts, the extension of the AUG-proximity effect is determined by the ORF sequence. Furthermore, we show how the mRNA secondary structure, which is affected by the ORF sequence, determines the AUG-proximity effect extension. Our data point out that the time taken to translate the short ORF, affected by its sequence and stability, besides being involved in modulating translation re-initiation, also plays an important role in establishing the extension of the AUG-proximity effect. | por |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1040 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Instituto Nacional de Saúde Doutor Ricardo Jorge, IP | por |
| dc.subject | Genómica Funcional e Estrutural | por |
| dc.title | How far ‘AUG-proximity effect’ goes? | por |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Tavira, Portugal | por |
| oaire.citation.title | FEBS International Workshop on “New Developments in RNA Biology", Sept 1- 4 2012 | por |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | por |