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Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal

2022-12-23Journal article Open access
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dc.contributor.authorBatista, Rita
dc.contributor.authorSaraiva, Margarida
dc.contributor.authorLopes, Teresa
dc.contributor.authorSilveira, Leonor
dc.contributor.authorCoelho, Anabela
dc.contributor.authorFurtado, Rosália
dc.contributor.authorCastro, Rita
dc.contributor.authorCorreia, Cristina Belo
dc.contributor.authorRodrigues, David
dc.contributor.authorHenriques, Pedro
dc.contributor.authorLóio, Sara
dc.contributor.authorSoeiro, Vanessa
dc.contributor.authorda Costa, Paulo Martins
dc.contributor.authorOleastro, Mónica
dc.contributor.authorPista, Angela
dc.date.accessioned2023-02-08T15:33:40Z
dc.date.available2023-02-08T15:33:40Z
dc.date.issued2022-12-23
dc.description.abstractBirds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence of the top three most important foodborne pathogens in free-living birds in Portugal, we investigated 108 individual fecal samples from free-living birds and one pooled sample of gull feces (n = 50) for the presence of Escherichia coli (pathogenic and non-pathogenic), Salmonella spp. and Campylobacter spp. Virulence- and antimicrobial resistance- (AMR) associated genes were detected by PCR and Whole-Genome Sequencing (WGS), and phenotypic (serotyping and AMR profiles) characterization was performed. Overall, 8.9% of samples tested positive for pathogenic E. coli, 2.8% for Salmonella spp., and 9.9% for Campylobacter spp. AMR was performed on all pathogenic isolates and in a fraction of non-pathogenic E. coli, being detected in 25.9% of them. Ten of the tested E. coli isolates were multidrug-resistant (MDR), and seven of them were Extended-spectrum β-lactamase (ESBL) producers. Among Salmonella (n = 3) and Campylobacter (n = 9), only one strain of C. jejuni was identified as MDR. Most of the identified serotypes/sequence types had already been found to be associated with human disease. These results show that free-living birds in Portugal may act as carriers of foodborne pathogens linked to human disease, some of them resistant to critically important antimicrobials.pt_PT
dc.description.sponsorshipThis work was supported by funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 773830: One Health European Joint Program, as part of the DiSCoVeR project (Discovering the sources of Salmonella, Campylobacter, VTEC and Antimicrobial Resistance). R.C. was the beneficiary of a fellowship from the same Programme on behalf of FedAMR project.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Environ Res Public Health. 2023,20(1):223. https://doi.org/10.3390/ ijerph20010223pt_PT
dc.identifier.doi10.3390/ijerph20010223pt_PT
dc.identifier.issn1661-7827
dc.identifier.urihttp://hdl.handle.net/10400.18/8514
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
dc.relation.publisherversionhttps://www.mdpi.com/1660-4601/20/1/223pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCampylobacter spp.pt_PT
dc.subjectSalmonella spp.pt_PT
dc.subjectPathogenic Escherichia coli;pt_PT
dc.subjectWhole-Genome Sequencingpt_PT
dc.subjectFree-living Birdspt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectPortugalpt_PT
dc.titleGenotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/773830/EU
oaire.citation.issue1pt_PT
oaire.citation.startPage223pt_PT
oaire.citation.titleInternational Journal of Environmental Research and Public Healthpt_PT
oaire.citation.volume20pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication94d118fb-33ce-49fa-b1ed-d5bddf63581d
relation.isProjectOfPublication.latestForDiscovery94d118fb-33ce-49fa-b1ed-d5bddf63581d

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