Publication
Effects and Mechanisms of Action of Preussin, a Marine Fungal Metabolite, against the Triple-Negative Breast Cancer Cell Line, MDA-MB-231, in 2D and 3D Cultures
| dc.contributor.author | Seabra, Rosária | |
| dc.contributor.author | Malhão, Fernanda | |
| dc.contributor.author | Correia, Alexandra | |
| dc.contributor.author | Costa, Carla | |
| dc.contributor.author | Kijjoa, Anake | |
| dc.contributor.author | Rocha, Eduardo | |
| dc.date.accessioned | 2024-02-08T15:37:03Z | |
| dc.date.available | 2024-02-08T15:37:03Z | |
| dc.date.issued | 2023-03 | |
| dc.description.abstract | Triple-negative breast cancer (TNBC) represents an aggressive subtype of breast cancer (BC) with a typically poorer prognosis than other subtypes of BC and limited therapeutic options. Therefore, new drugs would be particularly welcome to help treat TNBC. Preussin, isolated from the marine sponge-associated fungus, Aspergillus candidus, has shown the potential to reduce cell viability and proliferation as well as to induce cell death and cell cycle arrest in 2D cell culture models. However, studies that better mimic the tumors in vivo, such as 3D cell cultures, are needed. Here, we studied the effects of preussin in the MDA-MB-231 cell line, comparing 2D and 3D cell cultures, using ultrastructural analysis and the MTT, BrdU, annexin V-PI, comet (alkaline and FPG modified versions), and wound healing assays. Preussin was found to decrease cell viability, both in 2D and 3D cell cultures, in a dose-dependent manner, impair cell proliferation, and induce cell death, therefore excluding the hypothesis of genotoxic properties. The cellular impacts were reflected by ultrastructural alterations in both cell culture models. Preussin also significantly inhibited the migration of MDA-MB-231 cells. The new data expanded the knowledge on preussin actions while supporting other studies, highlighting its potential as a molecule or scaffold for the development of new anticancer drugs against TNBC. | pt_PT |
| dc.description.sponsorship | This research was partially supported by the strategic funding UIDB/04423/2020 and UIDP/04423/2020 through national funds provided by FCT—Fundação para a Ciência e a Tecnologia to CIIMAR. The Master in Oncology of the ICBAS—U.Porto offered additional support. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Mar Drugs. 2023 Mar 4;21(3):166. doi: 10.3390/md21030166. | pt_PT |
| dc.identifier.doi | 10.3390/md21030166 | pt_PT |
| dc.identifier.issn | 1660-3397 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/9057 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | Interdisciplinary Centre of Marine and Environmental Research | |
| dc.relation | Interdisciplinary Centre of Marine and Environmental Research | |
| dc.relation.publisherversion | https://www.mdpi.com/1660-3397/21/3/166 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | 3D Cell Culture | pt_PT |
| dc.subject | Cancer Cells Death | pt_PT |
| dc.subject | Cytotoxicity; Preussin | pt_PT |
| dc.subject | Triple-negative Breast Cancer | pt_PT |
| dc.subject | Toxicologia | pt_PT |
| dc.title | Effects and Mechanisms of Action of Preussin, a Marine Fungal Metabolite, against the Triple-Negative Breast Cancer Cell Line, MDA-MB-231, in 2D and 3D Cultures | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Interdisciplinary Centre of Marine and Environmental Research | |
| oaire.awardTitle | Interdisciplinary Centre of Marine and Environmental Research | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT | |
| oaire.citation.issue | 3 | pt_PT |
| oaire.citation.startPage | 166 | pt_PT |
| oaire.citation.title | Marine Drugs | pt_PT |
| oaire.citation.volume | 21 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.embargofct | Acesso de acordo com política editorial da revista. | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | e47e16ab-4259-4ce8-af09-551f7f31443a | |
| relation.isProjectOfPublication | c3064e67-096b-442c-b636-ae0e2da784a3 | |
| relation.isProjectOfPublication.latestForDiscovery | e47e16ab-4259-4ce8-af09-551f7f31443a |