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Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells

2021-08-09Journal article Open access
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dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorBrandão, Fátima
dc.contributor.authorCosta, Carla
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.date.accessioned2022-07-06T16:23:31Z
dc.date.available2022-07-06T16:23:31Z
dc.date.issued2021-08-09
dc.description(This article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health)pt_PT
dc.description.abstractStandard toxicity tests might not be fully adequate for evaluating nanomaterials since their unique features are also responsible for unexpected interactions. The in vitro cytokinesis-block micronucleus (CBMN) test is recommended for genotoxicity testing, but cytochalasin-B (Cyt-B) may interfere with nanoparticles (NP), leading to inaccurate results. Our objective was to determine whether Cyt-B could interfere with MN induction by TiO2 NP in human SH-SY5Y cells, as assessed by CBMN test. Cells were treated for 6 or 24 h, according to three treatment options: co-treatment with Cyt-B, post-treatment, and delayed co-treatment. Influence of Cyt-B on TiO2 NP cellular uptake and MN induction as evaluated by flow cytometry (FCMN) were also assessed. TiO2 NP were significantly internalized by cells, both in the absence and presence of Cyt-B, indicating that this chemical does not interfere with NP uptake. Dose-dependent increases in MN rates were observed in CBMN test after co-treatment. However, FCMN assay only showed a positive response when Cyt-B was added simultaneously with TiO2 NP, suggesting that Cyt-B might alter CBMN assay results. No differences were observed in the comparisons between the treatment options assessed, suggesting they are not adequate alternatives to avoid Cyt-B interference in the specific conditions tested.pt_PT
dc.description.sponsorshipThis research was funded by Ministerio de Ciencia e Innovación (PID2020-114908GA-I00), Xunta de Galicia (ED431B 2019/02); NanoBioBarriers (PTDC/MED-TOX/31162/2017) funded by Operational Program for Competitiveness and Internationalisation through European Regional Development Funds (FEDER/FNR) and through national funds by the Portuguese Foundation for Science and Technology (FCT); NanoLegaTox (PTDC/SAU-PUB/29651/2017) project co-financed by COMPETE 2020, Portugal 2020 and European Union, through FEDER; Ministerio de Educación, Cultura y Deporte (BEAGAL18/00142, to V.V.); FCT (SFRH/BD/101060/2014, to F.B.); and CA17140 NANO2CLINIC COST Action.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Mol Sci. 2021 Aug 9;22(16):8558. doi: 10.3390/ijms22168558pt_PT
dc.identifier.doi10.3390/ijms22168558pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.18/8077
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/22/16/8558pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSH-SY5Y Cellspt_PT
dc.subjectTiO2 Nanoparticlespt_PT
dc.subjectCytochalasin-Bpt_PT
dc.subjectCytokinesis-Block Micronucleus Testpt_PT
dc.subjectFlow Cytometry Micronucleus Testpt_PT
dc.subjectNanomaterialspt_PT
dc.subjectUptakept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleSuitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FSAU-PUB%2F29651%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F101060%2F2014/PT
oaire.citation.issue16pt_PT
oaire.citation.startPage8558pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume22pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1370de00-d228-47a7-8aed-320831dc4dcd
relation.isProjectOfPublication9f23e866-c585-4ebc-af90-ee03cf2d2655
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relation.isProjectOfPublication.latestForDiscovery8bcb6d0f-fda0-470a-906d-c67c257beedb

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