A study of quality assessment in clinical parasitology for detection of spiked specimens with Schistosoma mansoni

PNAEQ’s Parasitic Morphology of the National Health Institute Doutor Ricardo Jorge runs three External Quality Assessment (EQA) surveys per year, for microscopy-based detection of parasites. Human-infecting trematode, Schistosoma mansoni, widely occurs in geographical regions across sub-Saharan Africa, South American countries, the Caribbean and the Middle East. In order to mimic patient-like samples, S.mansoni extracted eggs from mice liver, were used to spike-in negative human faeces. Aims: Prepare negative stool specimens spiked with S. mansoni eggs for EQA testing; Evaluate the spiked sample homogeneity and stability; Assess the performance of participant laboratories.

Keywords

External Quality Assessment EQA Parasitic Morphology Schistosoma mansoni Avaliação Externa da Qualidade AEQ Programa Nacional de Avaliação Externa da Qualidade PNAEQ