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Genotoxicity evaluation of nanosized titanium dioxide, synthetic amorphous silica and multi-walled carbon nanotubes in human lymphocytes

2013-06-27Journal article Restricted access
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dc.contributor.authorTavares, Ana Maria
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorAntunes, Susana
dc.contributor.authorQuarré, S.
dc.contributor.authorSimar, S.
dc.contributor.authorTemmerman, P.D.
dc.contributor.authorVerleysen, E
dc.contributor.authorMast, J.
dc.contributor.authorJensen, K.A.
dc.contributor.authorNorppa, H.
dc.contributor.authorNesslany, F.
dc.contributor.authorSilva, Maria João
dc.date.accessioned2014-03-07T17:43:17Z
dc.date.available2014-03-07T17:43:17Z
dc.date.issued2013-06-27
dc.description.abstractToxicological characterization of manufactured nanomaterials (NMs) is essential for safety assessment, while keeping pace with innovation from their development and application in consumer products. The specific physicochemical properties of NMs, including size and morphology, might influence their toxicity and have impact on human health. The present work aimed to evaluate the genotoxicity of nanosized titanium dioxide (TiO2), synthetic amorphous silica (SAS) and multiwalled carbon nanotubes (MWCNT), in human lymphocytes. The morphology and size of those NMs were characterized by transmission electron microscopy, while the hydrodynamic particle size-distributions were determined by dynamic light scattering. Using a standardized procedure to ensure the dispersion of the NMs and the cytokinesis-block micronucleus assay (without metabolic activation), we observed significant increases in the frequencies of micronucleated binucleated cells (MNBC) for some TiO2 NMs and for two MWCNTs, although no clear dose–response relationships could be disclosed. In contrast, all forms of SAS analyzed in this study were unable to induce micronuclei. The present findings increase the weight of evidence towards a genotoxic effect of some forms of TiO2 and some MWCNT. Regarding safety assessment, the differential genotoxicity observed for closely related NMs highlights the importance of investigating the toxic potential of each NM individually, instead of assuming a common mechanism and equal genotoxic effects for a set of similar NMs.por
dc.identifier.citationToxicol In Vitro. 2014 Feb;28(1):60-9.Epub 2013 Jun 27por
dc.identifier.issn0887-2333
dc.identifier.otherdoi: http://dx.doi.org/10.1016/j.tiv.2013.06.009
dc.identifier.urihttp://hdl.handle.net/10400.18/1972
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier/ European Society for Toxicology in Vitropor
dc.relationAção Concertada Europeia NANOGENOTOX Grant Agreement No. 2009 21.por
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0887233313001604por
dc.subjectGenotoxicidade Ambientalpor
dc.subjectNanomaterialspor
dc.subjectGenotoxicitypor
dc.subjectHuman Cellspor
dc.subjectTitanium Dioxidepor
dc.subjectCarbon Nanotubespor
dc.subjectSynthetic amorphous silica nanomaterialspor
dc.subjectMultiwalled carbon nanotubespor
dc.subjectMicronucleus assaypor
dc.subjectPrimary human lymphocytespor
dc.subjectMorphology and sizepor
dc.titleGenotoxicity evaluation of nanosized titanium dioxide, synthetic amorphous silica and multi-walled carbon nanotubes in human lymphocytespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage69por
oaire.citation.startPage60por
oaire.citation.titleToxicology in Vitropor
oaire.citation.volume28(1)por
rcaap.rightsembargoedAccesspor
rcaap.typearticlepor

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