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Measurement of adsorption constants of laccase on gold nanoparticles to evaluate the enhancement in enzyme activity of adsorbed laccase

2018-06-20Journal article Restricted access
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dc.contributor.authorPeixoto de Almeida, Miguel
dc.contributor.authorQuaresma, Pedro
dc.contributor.authorSousa, Susana
dc.contributor.authorCouto, Cláudia
dc.contributor.authorGomes, Inês
dc.contributor.authorKrippahl, Ludwig
dc.contributor.authorFranco, Ricardo
dc.contributor.authorPereira, Eulália
dc.date.accessioned2019-03-29T11:41:16Z
dc.date.available2019-03-29T11:41:16Z
dc.date.issued2018-06-20
dc.descriptionSusana Sousa, Departamento de Doenças Infeciosas, Instituto Nacional de Saúde Doutor Ricardo Jorge, Porto, Portugalpt_PT
dc.description.abstractAdsorption of enzymes to nanoparticles and the mechanisms responsible for enzyme activity modulation of adsorbed enzymes are not well understood. In this work, gold nanoparticles were used for electrostatic adsorption of a plant-derived laccase. Adsorption constants were determined by four independent techniques: dynamic light scattering, electrophoretic light scattering, agarose gel electrophoresis and fluorescence quenching. Stable bionanoconjugates were formed with log K in the range 6.8-8.9. An increase in enzyme activity was detected, in particular at acidic and close to neutral pH values, a feature that expands the useful pH range of the enzyme. A model for the adsorption was developed, based on geometrical considerations and volume increase data from dynamic light scattering. This indicates that enzymes adsorbed to gold nanoparticles are ca. 9 times more active than the free enzyme.pt_PT
dc.description.sponsorshipMarta Giza is acknowledged for expert utilization of the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIGMS P41- GM103311). This work was supported by a) Unidade de Ciências Biomoleculares Aplicadas-UCIBIO which is financed by Portuguese national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728); and NOVA LINCS UID/CEC/04516/2013; and b) European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia), under the Partnership Agreement PT2020 through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE), Programa Operacional Regional do Norte (ON.2 - O Novo Norte), under the Quadro de Referência Estratégico Nacional (QREN) and funded by Fundo Europeu de Desenvolvimento Regional NORTE-01-0145-FEDER-000011. PQ, SS and IG acknowledge their post-doctoral fellowships (SFRH/BPD/84018/2012, SFRH/BPD/103788/2014 and SFRH/BPD/63850/2009, respectively), and MPA acknowledges his doctoral fellowship (SFRH/BD/95983/2013); all fellowships are financed by Fundação para a Ciência e a Tecnologia, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPhys Chem Chem Phys. 2018 Jun 20;20(24):16761-16769. doi: 10.1039/c8cp03116apt_PT
dc.identifier.doi10.1039/c8cp03116apt_PT
dc.identifier.issn1463-9076
dc.identifier.urihttp://hdl.handle.net/10400.18/6342
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relation.publisherversionhttps://pubs.rsc.org/en/Content/ArticleLanding/2018/CP/C8CP03116A#!divAbstractpt_PT
dc.subjectAdsorptionpt_PT
dc.subjectGoldpt_PT
dc.subjectHydrazonespt_PT
dc.subjectHydrogen-Ion Concentrationpt_PT
dc.subjectKineticspt_PT
dc.subjectLaccasept_PT
dc.subjectMetal Nanoparticlespt_PT
dc.subjectToxicodendronpt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleMeasurement of adsorption constants of laccase on gold nanoparticles to evaluate the enhancement in enzyme activity of adsorbed laccasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63850%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F95983%2F2013/PT
oaire.citation.endPage16769pt_PT
oaire.citation.issue24pt_PT
oaire.citation.startPage16761pt_PT
oaire.citation.titlePhysical Chemistry Chemical Physicspt_PT
oaire.citation.volume20pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica editorial da revistapt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication72797dcd-bef0-4686-8fd4-4ffc88f8cb5b
relation.isProjectOfPublicationd7883dc7-6335-4f26-8bd0-e2ea4bb07c07
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relation.isProjectOfPublicationd37e8520-44fe-4065-b003-15692e6e9d89
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