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Bridging the gap between PCR detection of Mycobacterium tuberculosis complex and tuberculosis diagnosis
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Autores
Orientador(es)
Resumo(s)
The growing demand for rapid diagnosis of tuberculosis
(TB) has led to the incorporation of nucleic acid amplification
(NAA) tests in case definitions. The objective of
this study was to evaluate the contribution of a real-time
polymerase chain reaction (PCR) assay in providing a
result predictive of a confirmed TB case. Respiratory
and extra-pulmonary specimens (n = 308) were subjected
to NAA, culture and smear microscopy. Qualitative PCR
assessment, translated by an increase in NAA cycles, disregarding
template copy number, resulted in an increase
in confirmed cases, helping to bridge the gap between
the test’s analytical performance and its actual performance
in TB diagnosis.
Descrição
Palavras-chave
Mycobacterium Tuberculosis Complex Real-time PCR Acid-fast Smear Microscopy diagnosis Tuberculosis Infecções Respiratórias
Contexto Educativo
Citação
Int J Tuberc Lung Dis. 2012;16(3):391-3. Epub 2012 Jan 5
Editora
International Union Against Tuberculosis and Lung Disease