Publication
Occupational second-hand smoke exposure: A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers
| datacite.subject.fos | Ciências Médicas::Ciências da Saúde | |
| datacite.subject.fos | Ciências Naturais::Ciências Biológicas | |
| dc.contributor.author | Neves, Sofia | |
| dc.contributor.author | Pacheco, Solange | |
| dc.contributor.author | Vaz, Fátima | |
| dc.contributor.author | James, Peter | |
| dc.contributor.author | Simões, Tânia | |
| dc.contributor.author | Penque, Deborah | |
| dc.contributor.editor | Neves | |
| dc.date.accessioned | 2025-02-18T15:11:38Z | |
| dc.date.available | 2025-02-18T15:11:38Z | |
| dc.date.issued | 2024-04-27 | |
| dc.description | Comparative Study | |
| dc.description.abstract | Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure. | eng |
| dc.description.abstract | Highlights: - Nasal airway epithelium is a valid tissue in the assessment of SHS-induced molecular changes; - HIF1α-glycolytic targets induced in cancer showed significant modulation in NSE; - NE may activate GSH metabolism and EEF2 – regulation in response to genotoxic stress; - FE may be at high risk for Serpin B3-associated diseases (lung cancer, asthma, COPD). | por |
| dc.description.sponsorship | This work was supported by the Calouste Gulbenkian Foundation, Portuguese Central Administration of Nacional Health System, the Portuguese Rede Nacional de Espectrometria de Massa, the National Institute of Health Doctor Ricardo Jorge, and the Centre for Toxicogenomics and Human Health – ToxOmics from NOVA Medical School-FCM, UNL. SN and SP were granted with PhD fellowships from Portuguese Fundação para a Ciência e a Tecnologia. | |
| dc.identifier.citation | Environ Toxicol Pharmacol . 2024 Jun:108:104459. doi: 10.1016/j.etap.2024.104459. Epub 2024 Apr 27 | |
| dc.identifier.doi | 10.1016/j.etap.2024.104459 | |
| dc.identifier.issn | 1382-6689 | |
| dc.identifier.pmid | 38685369 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10359 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S1382668924000991?via%3Dihub | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Cigarette Smoke | |
| dc.subject | Mass Spectrometry | |
| dc.subject | Nasal Epithelium | |
| dc.subject | Protein Network | |
| dc.subject | Proteomics | |
| dc.subject | Second-Hand Smoke | |
| dc.subject | Genómica Funcional e Estrutural | |
| dc.title | Occupational second-hand smoke exposure: A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 104459 | |
| oaire.citation.title | Environmental Toxicology and Pharmacology | |
| oaire.citation.volume | 108 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |