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Genetic diversity and population structure of Culex modestus across Europe: does recent appearance in the United Kingdom reveal a tendency for geographical spread?

2019-10-11Journal article Restricted access
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dc.contributor.authorHernández-Triana, L.M.
dc.contributor.authorBrugman, V.A.
dc.contributor.authorPramual, P.
dc.contributor.authorBarrero, E.
dc.contributor.authorNikolova, N.I.
dc.contributor.authorRuiz-Arrondo, I.
dc.contributor.authorKaiser, A.
dc.contributor.authorKrüger, A.
dc.contributor.authorLumley, S.
dc.contributor.authorOsório, H.C.
dc.contributor.authorIgnjatovic-Cupina, A.
dc.contributor.authorPetric, D.
dc.contributor.authorSetier-Rio, M.L.
dc.contributor.authorBodker, R.
dc.contributor.authorJohnson, N.
dc.date.accessioned2020-04-24T14:00:56Z
dc.date.available2020-04-24T14:00:56Z
dc.date.issued2019-10-11
dc.description.abstractIn mainland Europe, the mosquito species Culex modestus Ficalbi (1890) is a bridge vector for West Nile virus (WNV) from its natural bird-mosquito cycle to mammals. The present study assessed the genetic diversity of Cx. modestus, as well as related Culex species, using the mitochondrial COI DNA barcoding region and compared this with the population structure across Europe. A haplotype network was mapped to determine genealogical relationships among specimens. The intraspecific genetic diversity within individual Culex species was below 2%, whereas theinterspecificgeneticdivergencevariedfrom2.99%to13.74%.Intotal,76haplotypes were identified among 198 sequences. A median-joining network determined from 198 COI sequences identified two major lineages that were separated by at least four mutation steps. A high level of intraspecific genetic diversity was not detected in Cx. modestus in samples submitted from different European populations, which indicates that morphologically identified specimens represent a single species and not a species complex. Therefore, it is deduced that different populations of Cx. modestus will show a similar potential to transmit WNV, lending support to concerns that the population present in southeast England represents a risk of transmission to humans.pt_PT
dc.description.sponsorshipThe authors thank the Department for Environment and Rural Affairs (DEFRA), Scottish Government and Welsh Government (grants SV3045 and SE4113) for funding the present study. EU Framework Horizon 2020 Innovation Grant, European Virus Archive (EVAg, grant no. 653316) is also acknowledged for providing funds for sequencing. Ignacio Ruiz-Arrondo thanks the European Regional Development Fund (ERDF) for funding.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMed Vet Entomol. 2020 Mar;34(1):86-96. doi: 10.1111/mve.12412. Epub 2019 Oct 11pt_PT
dc.identifier.doi10.1111/mve.1241pt_PT
dc.identifier.issn0269-283X
dc.identifier.urihttp://hdl.handle.net/10400.18/6511
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley/ Royal Entomological Societypt_PT
dc.relationEuropean Virus Archive goes global
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1111/myc.13019pt_PT
dc.subjectCulicidaept_PT
dc.subjectGeneticdiversitypt_PT
dc.subjectPopulationstructurept_PT
dc.subjectCulexmodestuspt_PT
dc.subjectEuropept_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleGenetic diversity and population structure of Culex modestus across Europe: does recent appearance in the United Kingdom reveal a tendency for geographical spread?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEuropean Virus Archive goes global
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/653316/EU
oaire.citation.endPage96pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage86pt_PT
oaire.citation.titleMedical and Veterinary Entomologypt_PT
oaire.citation.volume34pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication3f53061f-588a-44da-8e2f-67a51cdee489
relation.isProjectOfPublication.latestForDiscovery3f53061f-588a-44da-8e2f-67a51cdee489

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