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Inter-laboratory variation in DNA damage using a standard comet assay protocol

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dc.contributor.authorForchhammer, Lykke
dc.contributor.authorErsson, Clara
dc.contributor.authorLoft, Steffen
dc.contributor.authorMoller, Lennart
dc.contributor.authorGodschalk, Roger W. L.
dc.contributor.authorvan Schooten, Frederik J
dc.contributor.authorJones, George D. D.
dc.contributor.authorHiggins, Jennifer A.
dc.contributor.authorCooke, Marcus
dc.contributor.authorMistry, Vilas
dc.contributor.authorKarbaschi, Mahsa
dc.contributor.authorCollins, Andrew R.
dc.contributor.authorAzqueta, Amaya
dc.contributor.authorPhillips, David H.
dc.contributor.authorSozeri, Osman
dc.contributor.authorRoutledge, Michael N.
dc.contributor.authorNelson-Smith, Kirsty
dc.contributor.authorRiso, Patrizia
dc.contributor.authorPorrini, Marisa
dc.contributor.authorMatullo, Giuseppe
dc.contributor.authorAllione, Alessandra
dc.contributor.authorStepnik, Maciej
dc.contributor.authorKomorowska, Magdalena
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorCosta, Solange
dc.contributor.authorCorcuera, Laura-Ana
dc.contributor.authorLopez de Cerain, Adela
dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorMoller, Peter
dc.date.accessioned2013-02-11T11:23:22Z
dc.date.available2013-02-11T11:23:22Z
dc.date.issued2012
dc.description.abstractThere are substantial inter-laboratory variations in the levels of DNA damage measured by the comet assay. The aim of this study was to investigate whether adherence to a standard comet assay protocol would reduce inter-laboratory variation in reported values of DNA damage. Fourteen laboratories determined the baseline level of DNA strand breaks (SBs)/alkaline labile sites and formamidopyrimidine DNA glycosylase (FPG)-sensitive sites in coded samples of mononuclear blood cells (MNBCs) from healthy volunteers. There were technical problems in seven laboratories in adopting the standard protocol, which were not related to the level of experience. Therefore, the inter-laboratory variation in DNA damage was only analysed using the results from laboratories that had obtained complete data with the standard comet assay protocol. This analysis showed that the differences between reported levels of DNA SBs/alkaline labile sites in MNBCs were not reduced by applying the standard assay protocol as compared with the laboratory's own protocol. There was large inter-laboratory variation in FPG-sensitive sites by the laboratory-specific protocol and the variation was reduced when the samples were analysed by the standard protocol. The SBs and FPG-sensitive sites were measured in the same experiment, indicating that the large spread in the latter lesions was the main reason for the reduced inter-laboratory variation. However, it remains worrying that half of the participating laboratories obtained poor results using the standard procedure. This study indicates that future comet assay validation trials should take steps to evaluate the implementation of standard procedures in participating laboratories.por
dc.identifier.citationMutagenesis. 2012 Nov;27(6):665-72. Epub 2012 Jul 27por
dc.identifier.issn0267-8357
dc.identifier.otherdoi: 10.1093/mutage/ges032
dc.identifier.urihttp://hdl.handle.net/10400.18/1213
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherOxford University Press (United Kingdom Environmental Mutagen Society)por
dc.relation.publisherversionhttp://mutage.oxfordjournals.org/content/27/6/665.abstractpor
dc.subjectDNA damagepor
dc.subjectComet Assaypor
dc.subjectInter-laboratory Variationpor
dc.subjectAr e Saúde Ocupacionalpor
dc.subjectGenotoxicidade Ambientalpor
dc.titleInter-laboratory variation in DNA damage using a standard comet assay protocolpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage672por
oaire.citation.startPage665por
oaire.citation.volume27(6)por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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