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Methylation of the miR-126 gene associated with glioma progression

2016-04Journal article Restricted access
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dc.contributor.authorCui, Hongwei
dc.contributor.authorMu, Yongping
dc.contributor.authorYu, Lei
dc.contributor.authorXi, Ya-guang
dc.contributor.authorMatthiesen, Rune
dc.contributor.authorSu, Xiulan
dc.contributor.authorSun, Wenjie
dc.date.accessioned2017-03-07T15:28:10Z
dc.date.available2021-05-01T00:30:12Z
dc.date.issued2016-04
dc.descriptionErratum in - Fam Cancer. 2016 Apr;15(2):325. doi: 10.1007/s10689-015-9848-2. https://link.springer.com/article/10.1007%2Fs10689-015-9848-2pt_PT
dc.description.abstractGliomas are the most common and the most malignant brain tumors, accouting for 45-55% of all intracranial tumors. The incidence of glioma worldwide is about 6-12 per 100,000. Recently, several studies showed that the activation of the oncogenes and the inactivation and/or loss of the tumor suppressor genes, especially for miRNA-21, let-7 and so on, are the most primary molecule event in gliomas. MicroRNAs (miRNAs) are a class of endogenously expressed small noncoding RNAs which are usually 21-23 nucleotides long. miRNAs regulate gene expression and play important roles in a variety of physiological and pathological processes, such as cell proliferation, differentiation and apoptosis. To date, Growing evidence has shown that mi RNAs are frequently dysregulated in human cancers and can act as both tumor suppressors and oncogenes. Along with the discovery of micro RNA, more and more research focusing on its relationship with glioma was carried out to investigate the biological features of glioma and to provide experimental evidence for glioma mechanism. In the present study, we aimed to verify the miRNA-126 down-regulation which showed in the results of glioma tissue miRNAs chip and discuss the miRNA-126 methylation in patients with glioma. A total of 50 samples from patients with glioma and 20 control samples from patients with cerebral trauma were included in this study. The expression levels of the miR-126 gene were detected using quantitative polymerase chain reaction (PCR), and the methylation status of miR-126 was examined using methylation-specific PCR-denaturing high-performance liquid chromatography (MSP-DHPLC). The expression level of miRNA-126 was found to be significantly higher in the control group (0.6134 ± 0.1214) than in the glioma group (0.2771 ± 0.1529; P < 0.05). The expression was also significantly elevated in low-grade gliomas (0.3117 ± 0.1474) compared with high-grade gliomas (0.1582 ± 0.1345; P < 0.05). In addition, increased methylation of miR-126 was found in 40% of glioma patients in our study (20/50 cases), resulting in significantly decreased miR-126 expression (0.1715 ± 0.1376; P < 0.05). Our results indicate that we verified successfully the miRNA-126 down-regulation phenomenon in patients with glioma which showed in the results of glioma tissue miRNAs chip and the miRNA-126 down-regulation through methylation in patients with glioma. So we could say that epigenetic modification is a crucial mechanism for controlling the expression of miR-126 in glioma.pt_PT
dc.description.sponsorshipThe research was supported by the college and university research program of the education department of Inner Mongolia Autonomous Region (No. NJZY13420).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFam Cancer. 2016 Apr;15(2):317-24. doi: 10.1007/s10689-015-9846-4pt_PT
dc.identifier.doi10.1007/s10689-015-9846-4pt_PT
dc.identifier.issn1389-9600
dc.identifier.urihttp://hdl.handle.net/10400.18/4546
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs10689-015-9846-4pt_PT
dc.subjectAdultpt_PT
dc.subjectAgedpt_PT
dc.subjectBrain Neoplasmspt_PT
dc.subjectCase-Control Studiespt_PT
dc.subjectDown-Regulationpt_PT
dc.subjectFemalept_PT
dc.subjectGene Expression Regulation, Neoplasticpt_PT
dc.subjectGenetic Association Studiespt_PT
dc.subjectGliomapt_PT
dc.subjectHumanspt_PT
dc.subjectMalept_PT
dc.subjectMethylationpt_PT
dc.subjectMicroRNAspt_PT
dc.subjectMiddle Agedpt_PT
dc.subjectPromoter Regions, Geneticpt_PT
dc.subjectReverse Transcriptase Polymerase Chain Reactionpt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.titleMethylation of the miR-126 gene associated with glioma progressionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage324pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage317pt_PT
oaire.citation.titleFamilial Cancerpt_PT
oaire.citation.volume15pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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