Repository logo
 
Publication

PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal

2020-12-04Journal article Open access
Simple item page
dc.contributor.authorCoelho, Catarina
dc.contributor.authorVieira-Pinto, Madalena
dc.contributor.authorVilares, Anabela
dc.contributor.authorGargaté, Maria João
dc.contributor.authorRodrigues, Manuela
dc.contributor.authorCardoso, Luís
dc.contributor.authorLopes, Ana Patrícia
dc.date.accessioned2021-04-05T15:56:06Z
dc.date.available2021-04-05T15:56:06Z
dc.date.issued2020-12-04
dc.descriptionThis article belongs to the Special Issue Parasitic Diseases from Wild Animals with Emphasis in Zoonotic Infectionspt_PT
dc.description.abstractWildlife plays an important role in the epidemiological cycle of Toxoplasma gondii. The European wild rabbit (Oryctolagus cuniculus) can be a source of infection to wild and domestic hosts, including human beings. Additionally, as an herbivorous animal, the European wild rabbit may also be a sentinel of environmental contamination with T. gondii and, consequently, an indicator of the potential transmission of this parasite. The purpose of the present work was to detect T. gondii DNA in European wild rabbit from central Portugal, as well as the possible implications for public health. Heart and diaphragm samples were obtained from 28 rabbits hunted in central Portugal. Nested PCR separately amplified the 5' and 3' ends of the surface antigen 2 (SAG2) gene. T. gondii DNA was detected in 19 out of the 28 sampled animals, resulting in a prevalence of 67.9%. These results show that T. gondii infection occurs in European wild rabbit and therefore may pose a potential risk for humans if consumed as raw or undercooked meat. Measures should be taken in order to prevent infection by this zoonotic parasite and for the conservation of wildlife. To the best of our knowledge, this is the first study performed by means of PCR on T. gondii in European wild rabbit meat samples.pt_PT
dc.description.sponsorshipThis research was funded by Fundação para a Ciência e Tecnologia (FCT), project UIDB/CVT/00772/2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2020 Dec 4;8(12):1926. doi: 10.3390/microorganisms8121926.pt_PT
dc.identifier.doi10.3390/microorganisms8121926pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/7636
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUIDB/CVT/00772/2020pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/8/12/1926pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEuropean Wild Rabbitpt_PT
dc.subjectOryctolagus cuniculuspt_PT
dc.subjectPortugalpt_PT
dc.subjectToxoplasma Gondiipt_PT
dc.subjectPolymerase Chain Reactionpt_PT
dc.subjectSurface Antigen 2pt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titlePCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12pt_PT
oaire.citation.startPage1926pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
32_PCR Detection.pdf
Size:
2.9 MB
Format:
Adobe Portable Document Format
Download

Collections

DDI - Artigos em revistas internacionais