Publication
Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella spp. Isolates from Pigs at Slaughterhouse and from Commercial Pork Meat in Portugal
| dc.contributor.author | Gonçalves, Carlota | |
| dc.contributor.author | Silveira, Leonor | |
| dc.contributor.author | Rodrigues, João | |
| dc.contributor.author | Furtado, Rosália | |
| dc.contributor.author | Ramos, Sónia | |
| dc.contributor.author | Nunes, Alexandra | |
| dc.contributor.author | Pista, Ângela | |
| dc.date.accessioned | 2025-03-20T15:19:49Z | |
| dc.date.available | 2025-03-20T15:19:49Z | |
| dc.date.issued | 2024-10-11 | |
| dc.description | (This article belongs to the Section Mechanism and Evolution of Antibiotic Resistance) | |
| dc.description.abstract | Background: Foodborne diseases are a serious public health concern, and food-producing animals are a major source of contamination. Methods: The present study analysed Escherichia coli and Salmonella spp. isolated from faecal samples of 100 fattening pigs and from 52 samples of pork meat. Results: The results showed that the majority of the analysed meat samples were considered satisfactory in terms of microbiological quality (92.3% for E. coli and 94.2% for Salmonella spp.). Salmonella spp. was identified in 5.8% of the meat samples, whereas E. coli was detected in 89.5% of all samples (69.2% in meat and 100% in faecal samples). Furthermore, 1.9% of the faecal samples contained Shiga-toxin-producing E. coli and 3.9% contained enterotoxigenic E. coli. All sequenced isolates presented virulence genes for extraintestinal pathogenic E. coli. Moreover, 75.0% of E. coli isolates from meat and 71.8% from faeces samples showed antibiotic resistance, with 40.7% and 51.4%, respectively, being multidrug-resistant (MDR). The most prevalent resistances were to tetracycline, ampicillin, and sulfamethoxazole, and one E. coli isolate showed resistance to extended-spectrum β-lactamase. Conclusions: This study highlights the role of pigs as a potential source of human contamination and the importance of a One Health approach to ensure food safety and to promote public health. | por |
| dc.description.sponsorship | This research was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No. 773830: One Health European Joint Programme, as part of the DiSCoVeR project (Discovering the sources of Salmonella, Campylobacter, VTEC and Antimicrobial Resistance), as well as developed under the project “FMVULusófona_ResisCampyOH”, funded by the Faculty of Veterinary Medicine from Lusófona University. | |
| dc.identifier.citation | Antibiotics (Basel). 2024 Oct 11;13(10):957. doi: 10.3390/antibiotics13100957 | |
| dc.identifier.doi | 10.3390/antibiotics13100957 | |
| dc.identifier.issn | 2079-6382 | |
| dc.identifier.pmid | 39452223 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10450 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| dc.relation.hasversion | https://www.mdpi.com/2079-6382/13/10/957 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | E. coli | |
| dc.subject | Salmonella | |
| dc.subject | Antimicrobial Resistance | |
| dc.subject | Whole-Genome Sequencing | |
| dc.subject | Food Safety | |
| dc.subject | Pork | |
| dc.subject | Infecções Gastrointestinais | |
| dc.subject | Segurança Alimentar | |
| dc.subject | Resistência aos Antimicrobianos | |
| dc.subject | Portugal | |
| dc.title | Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella spp. Isolates from Pigs at Slaughterhouse and from Commercial Pork Meat in Portugal | por |
| dc.type | research article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/773830/EU | |
| oaire.citation.issue | 10 | |
| oaire.citation.startPage | 957 | |
| oaire.citation.title | Antibiotics | |
| oaire.citation.volume | 13 | |
| oaire.fundingStream | H2020 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.name | European Commission | |
| relation.isProjectOfPublication | 94d118fb-33ce-49fa-b1ed-d5bddf63581d | |
| relation.isProjectOfPublication.latestForDiscovery | 94d118fb-33ce-49fa-b1ed-d5bddf63581d |